The reported prevalence of naturally occurring anti–dog erythrocyte antigen (DEA) 7 antibodies in DEA 7–negative dogs is as high as 50%. Characterization of these antibodies may better define their importance in canine transfusion medicine. We determined in vitro activity, specificity, and titer of anti–DEA 7 antibodies in DEA 7–negative dogs. Plasma samples from 317 DEA 7–negative dogs were cross-matched with DEA 7–positive red blood cells (RBCs) using gel column technology. Agglutination occurred with DEA 7–positive RBCs but not with DEA 7–negative RBCs in 73 samples (23%), which were hence classified as containing anti–DEA 7 antibodies. These samples were evaluated for hemolytic and agglutinating activity, strength of agglutination, and antibody specificity and titers. All samples showed agglutination but none showed hemolysis. Gel agglutination was graded as 1+ for 20 samples (27%), 2+ for 49 samples (67%), 3+ for 4 samples (6%); no samples were graded 4+. The agglutination titer was <1:2 for 51 samples (73%), 1:2 for 13 samples (19%), 1:4 for 4 samples (5%), and 1:8 for 2 samples (3%). Of 16 samples treated with 2-mercaptoethanol, 11 samples (69%) contained only IgM, 4 samples (25%) exhibited only IgG activity, and 1 sample (6%) had both IgG and IgM activity. Low titers of warm, weakly agglutinating, mostly naturally occurring IgM anti–DEA 7 antibodies were found in 23% of DEA 7–negative dogs. The presence of naturally occurring anti–DEA 7 antibodies suggests that cross-matching of canine blood recipients is advisable, even at first transfusion, to minimize delayed transfusion reactions.

Activity, specificity, and titer of naturally occurring canine anti-DEA 7 antibodies / E. Spada, D. Proverbio, L. Baggiani, I. Canzi, R. Perego. - In: JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION. - ISSN 1040-6387. - 28:6(2016), pp. 705-708.

Activity, specificity, and titer of naturally occurring canine anti-DEA 7 antibodies

E. Spada
Primo
Conceptualization
;
D. Proverbio
Secondo
;
R. Perego
Ultimo
2016

Abstract

The reported prevalence of naturally occurring anti–dog erythrocyte antigen (DEA) 7 antibodies in DEA 7–negative dogs is as high as 50%. Characterization of these antibodies may better define their importance in canine transfusion medicine. We determined in vitro activity, specificity, and titer of anti–DEA 7 antibodies in DEA 7–negative dogs. Plasma samples from 317 DEA 7–negative dogs were cross-matched with DEA 7–positive red blood cells (RBCs) using gel column technology. Agglutination occurred with DEA 7–positive RBCs but not with DEA 7–negative RBCs in 73 samples (23%), which were hence classified as containing anti–DEA 7 antibodies. These samples were evaluated for hemolytic and agglutinating activity, strength of agglutination, and antibody specificity and titers. All samples showed agglutination but none showed hemolysis. Gel agglutination was graded as 1+ for 20 samples (27%), 2+ for 49 samples (67%), 3+ for 4 samples (6%); no samples were graded 4+. The agglutination titer was <1:2 for 51 samples (73%), 1:2 for 13 samples (19%), 1:4 for 4 samples (5%), and 1:8 for 2 samples (3%). Of 16 samples treated with 2-mercaptoethanol, 11 samples (69%) contained only IgM, 4 samples (25%) exhibited only IgG activity, and 1 sample (6%) had both IgG and IgM activity. Low titers of warm, weakly agglutinating, mostly naturally occurring IgM anti–DEA 7 antibodies were found in 23% of DEA 7–negative dogs. The presence of naturally occurring anti–DEA 7 antibodies suggests that cross-matching of canine blood recipients is advisable, even at first transfusion, to minimize delayed transfusion reactions.
alloantibodies; canine transfusion medicine; dog erythrocyte antigen 7
Settore VET/08 - Clinica Medica Veterinaria
Settore VET/03 - Patologia Generale e Anatomia Patologica Veterinaria
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2434/437399
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