Background: Endometritis reduces fertility and is responsible for major economic losses in beef and dairy industries. The aim of this study was to evaluate an alternative therapy using platelet-rich plasma (PRP). PRP was tested in vivo, after bovine intrauterine administration, and in vitro on endometrial cells. Methods: Bovine endometrial cells were cultured until passage (P) 10 with 5% or 10% PRP. Effect of PRP on endometrial cell proliferation and on the expression of genes [prostaglandin-endoperoxide synthase 2 (COX2), tumor protein p53 (TP53), oestrogen receptors (ER-α and ER-β), progesterone receptor (PR) and c-Myc] involved in the regulation of oestrus cycle and fetal-maternal interaction were evaluated. Moreover, to evaluate the ability of PRP to counteract inflammation, 10 and 100 ng/ml of bacterial endotoxin lipopolysaccharide (LPS) were used to inflame endometrial cells in vitro for 1, 6, 12, 24 and 48 h. The expression of genes such as interleukin 1β (IL-1β), interleukin-8 (IL-8), inducible nitric oxide synthase (iNOS), prostaglandin-endoperoxide synthase 2 (COX2/PTGS2), and the release of PGE-2, IL-1β and IL-8 were evaluated. Results: In vivo treatment with PRP increased the detection of PR. In vitro, 5% PRP at passage 5 increased proliferation rate and induced a significant increase in the expression of all studied genes. Furthermore, the results revealed that 10 ng/ml of LPS is the most effective dose to obtain an inflammatory response, and that PRP treatment significantly down regulated the expression of pro-inflammatory genes. Conclusion: This study lays the foundations for the potential treatment of endometritis with PRP in vivo.
Effects of platelet-rich plasma in a model of bovine endometrial inflammation in vitro / M.G. Marini, C. Perrini, P. Esposti, B. Corradetti, D. Bizzaro, P. Riccaboni, E. Fantinato, G. Urbani, G. Gelati, F. Cremonesi, A. Lange-Consiglio. - In: REPRODUCTIVE BIOLOGY AND ENDOCRINOLOGY. - ISSN 1477-7827. - 14(2016 Sep 13), pp. 58.1-58.17.
|Titolo:||Effects of platelet-rich plasma in a model of bovine endometrial inflammation in vitro|
PERRINI, CLAUDIA (Secondo)
CREMONESI, FAUSTO (Penultimo)
LANGE CONSIGLIO, ANNA (Ultimo)
|Parole Chiave:||Cattle; Endometrial cells; Platelet-rich plasma; LPS; gene expression|
|Settore Scientifico Disciplinare:||Settore VET/10 - Clinica Ostetrica e Ginecologia Veterinaria|
|Data di pubblicazione:||13-set-2016|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1186/s12958-016-0195-4|
|Appare nelle tipologie:||01 - Articolo su periodico|