A high-performance liquid chromatographic method for the determination of total plasma homocyst(e)ine [H(e)]after reduction with sodium tetrahydroborate and precolumn derivatization with o-phthaldialdehyde is described. The analyses, carried out on a reversed-phase C18 column, were based on spectrofluorimetric detection. The sensitivity was 1 pmol per injection and the intra- and inter-assay relative standard deviations were 1.8% and 5%, respectively. The plasma H(e) concentration determined in 40 healthy volunteers (20-60 years old) was 12.4±2.9 μM (mean±S.D.), in good agreement with reference values.
High-performance liquid chromatographic method with fluorescence detection for the determination of total homocyst(e)ine in plasma / I. Fermo, C. Arcelloni, E. De Vecchi, S. Vigano', R. Paroni. - In: JOURNAL OF CHROMATOGRAPHY. - ISSN 0021-9673. - 593:1-2(1992), pp. 171-176.
High-performance liquid chromatographic method with fluorescence detection for the determination of total homocyst(e)ine in plasma
R. ParoniUltimo
1992
Abstract
A high-performance liquid chromatographic method for the determination of total plasma homocyst(e)ine [H(e)]after reduction with sodium tetrahydroborate and precolumn derivatization with o-phthaldialdehyde is described. The analyses, carried out on a reversed-phase C18 column, were based on spectrofluorimetric detection. The sensitivity was 1 pmol per injection and the intra- and inter-assay relative standard deviations were 1.8% and 5%, respectively. The plasma H(e) concentration determined in 40 healthy volunteers (20-60 years old) was 12.4±2.9 μM (mean±S.D.), in good agreement with reference values.
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