Mesenchymal stem cells (MSC) isolated from bone marrow (BM) and umbilical cord of human, canine, guinea pig, and rat have been shown to differentiate into several tissues. The aim of this work was to investigate the possibility of isolating pluripotent precursor cells from equine BM and compare them with umbilical cord matrix cells and amniotic epithelial cells. BM samples were obtained from adult horses by Histopaque gradient centrifugation and cultured in monolayer. Adherent fibroblast-like cells were tested for their stemness by known specific markers. Evidence for MCS lineage was made via reverse transcription-PCR. After adding EGF, horse BM-MSCs adopted a fibroblast-like morphology and, as expected, expressed Sox-2, Oct-4 and Nanog. They did not expressed a negative marker such as CD34. GADPH expression was used as internal control. Umbilical cord cells showed a similar morphology

Isolamento, coltura in vitro e caratterizzazione di cellule staminali da cordone ombelicale di puledro alla nascita / F. Cremonesi, S. Violini, A. Lange Consiglio, P. Ramelli, G. Ranzenigo, P. Mariani. - In: ATTI DELLA SOCIETÀ ITALIANA DELLE SCIENZE VETERINARIE. - ISSN 1825-4454. - 61:(2007), pp. 33-34. (Intervento presentato al 61. convegno Convegno Nazionale tenutosi a Salsomaggiore Terme (PR) nel 2007).

Isolamento, coltura in vitro e caratterizzazione di cellule staminali da cordone ombelicale di puledro alla nascita

F. Cremonesi
Primo
;
A. Lange Consiglio;G. Ranzenigo
Penultimo
;
2007

Abstract

Mesenchymal stem cells (MSC) isolated from bone marrow (BM) and umbilical cord of human, canine, guinea pig, and rat have been shown to differentiate into several tissues. The aim of this work was to investigate the possibility of isolating pluripotent precursor cells from equine BM and compare them with umbilical cord matrix cells and amniotic epithelial cells. BM samples were obtained from adult horses by Histopaque gradient centrifugation and cultured in monolayer. Adherent fibroblast-like cells were tested for their stemness by known specific markers. Evidence for MCS lineage was made via reverse transcription-PCR. After adding EGF, horse BM-MSCs adopted a fibroblast-like morphology and, as expected, expressed Sox-2, Oct-4 and Nanog. They did not expressed a negative marker such as CD34. GADPH expression was used as internal control. Umbilical cord cells showed a similar morphology
Puledro ; cordone ombelicale ; cellule staminali
Settore VET/10 - Clinica Ostetrica e Ginecologia Veterinaria
Settore VET/01 - Anatomia degli Animali Domestici
2007
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/42670
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