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EnglishNeo-vascularization is a key factor in tissue regeneration within porous scaffolds. Here, we tested the hypothesis that micro-patterned scaffolds, with precisely-designed, open micro-channels, might help endothelial cells to produce intra-scaffold vascular networks. Three series of micro-patterned scaffolds were produced via electrochemical replica-deposition of chitosan and cross-linking. All had regularly-oriented micro-channels (φ 500 μm), which differed for the inter-channel spacing, at 600, 700, or 900 μm, respectively. Random-pore scaffolds, using the same technique, were taken as controls. Physical-mechanical characterization revealed high water uptake and favorable elastic mechanical behavior for all scaffolds, slightly reduced in the presence of cross-linking and enhanced with the 700 μm-spaced micro-pattern. At MTT assay, mouse endothelial cell viability was >90% at day 1, 3 and 7, confirmed by visual examination with scanning electron microscopy (SEM). Intra-scaffold cell density, at fluorescence analysis, was higher for the 600 μm-spaced and the 700 μm-spaced micro-patterns over the others. The 700 μm-spaced scaffold was selected for the in vivo testing, to be compared to the random-pore one. Neither type produced an inflammatory reaction; both showed excellent tissue ingrowth. Micro-patterned scaffolds enhanced neo-vascularization, demonstrated by immunofluorescent, semi-quantitative analyses. These findings support the use of micro-patterned porous scaffolds, with adequately spaced micro-channels, to promote neo-vascularization.
Hierarchic micro-patterned porous scaffolds via electrochemical replica-deposition enhance neo-vascularization / E.M. Varoni, L. Altomare, A. Cochis, A. Ghalayaniesfahani, A. Cigada, L. Rimondini, L. De Nardo. - In: BIOMEDICAL MATERIALS. - ISSN 1748-6041. - 11:2(2016), pp. 025018.1-025018.13.
| Titolo: | Hierarchic micro-patterned porous scaffolds via electrochemical replica-deposition enhance neo-vascularization |
| Autori: | |
| Parole Chiave: | neovascularization; electrochemical deposition; chitosan; tissue engineering |
| Settore Scientifico Disciplinare: | Settore ING-IND/22 - Scienza e Tecnologia dei Materiali |
| Data di pubblicazione: | 2016 |
| Rivista: | |
| Tipologia: | Article (author) |
| Digital Object Identifier (DOI): | http://dx.doi.org/10.1088/1748-6041/11/2/025018 |
| Appare nelle tipologie: | 01 - Articolo su periodico |
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