The fully unprotected alpha-C-glycosyl analogue of N-acetylgalactosamine 9 was conjugated by a non-natural oxime bond to the segment peptides (328--340)OVA and (327--339)OVA, affording neoglycopeptides 1--2 and 3, having one or two sugar units, respectively. The three neoglycopeptides were tested in vitro in an antigen presentation assay as antitumor vaccines. Neoglycopeptides 1--3 could be presented to and recognized by the T cell receptor; neoglycopeptide 3, bearing two B-epitopes, was presented to the TCR with higher efficiency, compared to neoglycopeptide 2, having only one B-epitope.
|Titolo:||Novel Tn antigen-containing neoglycopeptides: synthesis and evaluation as anti tumor vaccines|
RESCIGNO, MARIA (Secondo)
|Parole Chiave:||chemoselective ligation reactions; active specific immunotherapy; carbon-linked glycopeptides; mouse dendritic cells; solid-phase synthesis; C-glycoside analog; stereoselective-synthesis; carbohydrate antigens; N-acetygalactosamine; anticancer vaccines|
|Settore Scientifico Disciplinare:||Settore MED/04 - Patologia Generale|
|Data di pubblicazione:||mag-2002|
|Digital Object Identifier (DOI):||10.1016/S0968-0896(01)00433-3|
|Appare nelle tipologie:||01 - Articolo su periodico|