Fertilization in flowering plants requires a complex series of coordinated events involving interaction between the male and female gametophyte. We report here molecular data on one of the key events underpinning this process - the death of the receptive synergid cell and the coincident bursting of the pollen tube inside the ovule to release the sperms.We show that two REM transcription factors, VALKYRIE (VAL) and VERDANDI (VDD), both targets of the ovule identity MADS-box complex SEEDSTICK-SEPALLATA3, interact to control the death of the receptive synergid cell. In vdd_1/+ mutants and VAL_RNAi lines we find that GAMETOPHYTIC FACTOR 2 (GFA2), required for synergid degeneration, is down regulated, while FERONIA (FER) and MYB98 expression, necessary for pollen tube attraction and perception remain unaffected. We also demonstrate that the vdd_1/+ phenotype can be rescued by expressing VDD or GFA2 in the synergid cells. Taken together, our findings reveal that the death of the receptive synergid cell is essential for the maintenance of the following generations, and that a complex formed of VDD and VAL regulate this event.
Live and let die : a REM complex promotes fertilization through synergid cell death in Arabidopsis / M.A. Mendes, R. Guerra, B. Castelnovo, Y.S. Velazquez, P. Morandini, S. Manrique Urpì, N. Baumann, R. Groß Hardt, H. Dickinson, L. Colombo. - In: DEVELOPMENT. - ISSN 0950-1991. - 143:15(2016 Aug 01), pp. 2780-2790.
Titolo: | Live and let die : a REM complex promotes fertilization through synergid cell death in Arabidopsis |
Autori: | MIRANDA MENDES, MARTA ADELINA (Primo) GUERRA, ROSALINDA (Secondo) COLOMBO, LUCIA (Ultimo) |
Settore Scientifico Disciplinare: | Settore BIO/01 - Botanica Generale Settore BIO/04 - Fisiologia Vegetale |
Data di pubblicazione: | 1-ago-2016 |
Rivista: | |
Tipologia: | Article (author) |
Data ahead of print / Data di stampa: | 23-giu-2016 |
Digital Object Identifier (DOI): | http://dx.doi.org/10.1242/dev.134916 |
Appare nelle tipologie: | 01 - Articolo su periodico |
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File | Descrizione | Tipologia | Licenza | |
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Mendes(2016)REM.pdf | Articolo principale | Post-print, accepted manuscript ecc. (versione accettata dall'editore) | Open Access Visualizza/Apri | |
2780.full.pdf | Publisher's version/PDF | Open Access Visualizza/Apri |