The aim of this study was to evaluate probiotic characteristics of Lactobacillus salivarius LS01 and Bifidobacterium breve BR03 alone and in combination and their immunomodulatory activity in asthmatic subjects. Subjects affected by allergic asthma were recruited. Initially, LS01 and BR03 were analyzed for their growth compatibility by a broth compatibility assay. To study the antimicrobial activity of probiotic strains, an agar diffusion assay was performed. Finally, cytokine production by peripheral blood mononuclear cells (PBMCs) stimulated with LS01 and BR03 was determined by means of specific quantitative enzyme-linked immunosorbent assay (ELISA). The growth of some clinical pathogens were slightly inhibited by LS01 and LS01-BR03 co-culture supernatant not neutralized to pH 6.5, while only the growth of E. coli and S. aureus was inhibited by the supernatant of LS01 and LS01-BR03 neutralized to pH 6.5. Furthermore, LS01 and BR03 combination was able to decrease the secretion of proinflammatory cytokines by PBMCs, leading to an intense increase in IL-10 production. L. salivarius LS01 and B. breve BR03 showed promising probiotic properties and beneficial immunomodulatory activity that are increased when the 2 strains are used in combination in the same formulation.

Immunomodulatory effects of Lactobacillus salivarius LS01 and Bifidobacterium breve BR03, alone and in combination, on peripheral blood mononuclear cells of allergic asthmatics / L. Drago, E. De Vecchi, A. Gabrieli, R. De Grandi, M. Toscano. - In: ALLERGY, ASTHMA & IMMUNOLOGY RESEARCH. - ISSN 2092-7355. - 7:4(2015), pp. 409-413. [10.4168/aair.2015.7.4.409]

Immunomodulatory effects of Lactobacillus salivarius LS01 and Bifidobacterium breve BR03, alone and in combination, on peripheral blood mononuclear cells of allergic asthmatics

L. Drago
Primo
;
E. De Vecchi
Secondo
;
A. Gabrieli;M. Toscano
Ultimo
2015

Abstract

The aim of this study was to evaluate probiotic characteristics of Lactobacillus salivarius LS01 and Bifidobacterium breve BR03 alone and in combination and their immunomodulatory activity in asthmatic subjects. Subjects affected by allergic asthma were recruited. Initially, LS01 and BR03 were analyzed for their growth compatibility by a broth compatibility assay. To study the antimicrobial activity of probiotic strains, an agar diffusion assay was performed. Finally, cytokine production by peripheral blood mononuclear cells (PBMCs) stimulated with LS01 and BR03 was determined by means of specific quantitative enzyme-linked immunosorbent assay (ELISA). The growth of some clinical pathogens were slightly inhibited by LS01 and LS01-BR03 co-culture supernatant not neutralized to pH 6.5, while only the growth of E. coli and S. aureus was inhibited by the supernatant of LS01 and LS01-BR03 neutralized to pH 6.5. Furthermore, LS01 and BR03 combination was able to decrease the secretion of proinflammatory cytokines by PBMCs, leading to an intense increase in IL-10 production. L. salivarius LS01 and B. breve BR03 showed promising probiotic properties and beneficial immunomodulatory activity that are increased when the 2 strains are used in combination in the same formulation.
No
English
Asthma; Immune system; Probiotics; Immunology and Allergy; Immunology; Pulmonary and Respiratory Medicine
Settore MED/07 - Microbiologia e Microbiologia Clinica
Articolo
Esperti anonimi
Pubblicazione scientifica
2015
Korean Academy of Asthma, Allergy and Clinical Immunology
7
4
409
413
5
Pubblicato
Periodico con rilevanza internazionale
Aderisco
info:eu-repo/semantics/article
Immunomodulatory effects of Lactobacillus salivarius LS01 and Bifidobacterium breve BR03, alone and in combination, on peripheral blood mononuclear cells of allergic asthmatics / L. Drago, E. De Vecchi, A. Gabrieli, R. De Grandi, M. Toscano. - In: ALLERGY, ASTHMA & IMMUNOLOGY RESEARCH. - ISSN 2092-7355. - 7:4(2015), pp. 409-413. [10.4168/aair.2015.7.4.409]
reserved
Prodotti della ricerca::01 - Articolo su periodico
5
262
Article (author)
no
L. Drago, E. De Vecchi, A. Gabrieli, R. De Grandi, M. Toscano
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/424991
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