Background: Influenza-like illness (ILI) is a cause of child morbidity during the winter. Predominantly caused by influenzavirus, it can also be caused by different respiratory viruses, such as enterovirus (EV) and human parechovirus (HPeV). Aim: to describe the circulation of influenzaviruses, HPeV, EV and EV-D68 in children aged up to 5 years during the five previous winter seasons (2010/11-2014/15) in Lombardy (Northern Italy). Methods: Throat swabs were collected from 392 children (0-5 years; 45.4% females, median age: 3 years ; IQR: 4 years) with ILI in the framework of the Italian influenza surveillance network InfluNet. RNA was extracted from each sample (Invitek), and amplified for detection of influenzavirus type A/B and EV/HPeV with two one-step multiplex real-time RT-PCRs. EV-positive samples were tested for EV-D68 with a one-step real-time RT-PCR. Results: Of the 392 samples, 46.9% (184/392) were influenzavirus-positive (75% type A), 9.4% were EV-positive and 3.8% were HPeV-positive. EV-D68 was not detected. Among the 208 influenzavirus-negative samples, 11.5% and 3.8% were positive for EV and HPeV, respectively. HPeV-positive children were younger (mean age: 21 months) than both EV-positive (mean: 34 months, p=0.002) and influenzavirus-positive children (mean: 39 months, p<0.001). HPeV was identified in 1.6% (in 2010/11) to 5% (in 2014/15) of samples. EV was similarly detected (9.7% to 11.3%) except in 2012/13 (4.3%). Co-infections were detected in 10.9% (20/184) of cases: 7.1% (13/184) were influenzavirus/EV-positive and 3.9% were influenzavirus/HPeV-positive. None were EV/HPeV. Conclusions: Almost half (47%) of ILI cases in our study were influenzavirus-positive, as expected. No EV-D68 was identified. Of the remaining influenzavirus-negative cases, 15% were positive for EV or HPeV, highlighting the importance of monitoring the circulation of these viruses, generally not included among common investigated pathogens.

Circulation of enteroviruses and human parechoviruses in children aged up to 5 years with influenza-like illness in Northern Italy during the 5 previous winter seasons / L. Bubba, L. Pellegrinelli, M. Martinelli, T. Raphael, M. Lauréti, S. Binda, E. Pariani. ((Intervento presentato al convegno ESCAIDE2015 tenutosi a Stockholm nel 2015.

Circulation of enteroviruses and human parechoviruses in children aged up to 5 years with influenza-like illness in Northern Italy during the 5 previous winter seasons

L. Bubba
Primo
;
L. Pellegrinelli
Secondo
;
M. Martinelli;S. Binda
Penultimo
;
E. Pariani
Ultimo
2015-11

Abstract

Background: Influenza-like illness (ILI) is a cause of child morbidity during the winter. Predominantly caused by influenzavirus, it can also be caused by different respiratory viruses, such as enterovirus (EV) and human parechovirus (HPeV). Aim: to describe the circulation of influenzaviruses, HPeV, EV and EV-D68 in children aged up to 5 years during the five previous winter seasons (2010/11-2014/15) in Lombardy (Northern Italy). Methods: Throat swabs were collected from 392 children (0-5 years; 45.4% females, median age: 3 years ; IQR: 4 years) with ILI in the framework of the Italian influenza surveillance network InfluNet. RNA was extracted from each sample (Invitek), and amplified for detection of influenzavirus type A/B and EV/HPeV with two one-step multiplex real-time RT-PCRs. EV-positive samples were tested for EV-D68 with a one-step real-time RT-PCR. Results: Of the 392 samples, 46.9% (184/392) were influenzavirus-positive (75% type A), 9.4% were EV-positive and 3.8% were HPeV-positive. EV-D68 was not detected. Among the 208 influenzavirus-negative samples, 11.5% and 3.8% were positive for EV and HPeV, respectively. HPeV-positive children were younger (mean age: 21 months) than both EV-positive (mean: 34 months, p=0.002) and influenzavirus-positive children (mean: 39 months, p<0.001). HPeV was identified in 1.6% (in 2010/11) to 5% (in 2014/15) of samples. EV was similarly detected (9.7% to 11.3%) except in 2012/13 (4.3%). Co-infections were detected in 10.9% (20/184) of cases: 7.1% (13/184) were influenzavirus/EV-positive and 3.9% were influenzavirus/HPeV-positive. None were EV/HPeV. Conclusions: Almost half (47%) of ILI cases in our study were influenzavirus-positive, as expected. No EV-D68 was identified. Of the remaining influenzavirus-negative cases, 15% were positive for EV or HPeV, highlighting the importance of monitoring the circulation of these viruses, generally not included among common investigated pathogens.
Influenzavirus; enterovirus; parechovirus; influenza-like illness,enterovirus-D68
Settore MED/42 - Igiene Generale e Applicata
Circulation of enteroviruses and human parechoviruses in children aged up to 5 years with influenza-like illness in Northern Italy during the 5 previous winter seasons / L. Bubba, L. Pellegrinelli, M. Martinelli, T. Raphael, M. Lauréti, S. Binda, E. Pariani. ((Intervento presentato al convegno ESCAIDE2015 tenutosi a Stockholm nel 2015.
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2434/423661
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