Estrogen receptors (ER) are known to play an important regulatory role in mammary gland development as well as in its neoplastic transformation. Although several studies highlighted the contribution of ER signaling in the breast transformation, little is known about the dynamics of ER state of activity during carcinogenesis due to the lack of appropriate models for measuring the extent of receptor signaling in time, in the same animal. To this aim, we have developed a reporter mouse model for the non-invasive in vivo imaging of ER activity: the ERE-Luc reporter mouse. ERE-Luc is a transgenic mouse generated with a firefly luciferase (Luc) reporter gene driven by a minimal promoter containing an estrogen responsive element (ERE). This model allows to measure receptor signaling in longitudinal studies by bioluminescence imaging (BLI). Here, we have induced sporadic mammary cancers by treating systemically ERE-Luc reporter mice with DMBA (9,10-dimethyl 1,2-benzanthracene) and measured receptor signaling by in vivo imaging in individual animals from early stage until a clinically palpable tumor appeared in the mouse breast. We showed that DMBA administration induces an increase of bioluminescence in the whole abdominal area 6 h after treatment, the signal rapidly disappears. Several weeks later, strong bioluminescence is observed in the area corresponding to the mammary glands. In vivo and ex vivo imaging analysis demonstrated that this bioluminescent signal is localized in the breast area undergoing neoplastic transformation. We conclude that this non-invasive assay is a novel relevant tool to identify the activation of the ER signaling prior the morphological detection of the neoplastic transformation.
Bioluminescence imaging of estrogen receptor activity during breast cancer progression / C. Vantaggiato, G. Dell'Omo, B. Ramachandran, I. Manni, E. Radaelli, E. Scanziani, G. Piaggio, A. Maggi, P. Ciana. - In: AMERICAN JOURNAL OF NUCLEAR MEDICINE AND MOLECULAR IMAGING. - ISSN 2160-8407. - 6:1(2016), pp. 32-41.
|Titolo:||Bioluminescence imaging of estrogen receptor activity during breast cancer progression|
DELL'OMO, GIULIA (Secondo)
MAGGI, ADRIANA CATERINA (Penultimo)
CIANA, PAOLO (Ultimo)
|Parole Chiave:||mammary tumorigenesis; chemical carcinogenesis; hormone-dependent cancer; optical imaging|
|Settore Scientifico Disciplinare:||Settore BIO/14 - Farmacologia|
|Data di pubblicazione:||2016|
|Appare nelle tipologie:||01 - Articolo su periodico|