3ovine viral diarrhea virus (BVDV) and bovine herpesvirus 1 (BHV-1) are pathogens that infect immunocompetent cells and can display a devastating ímpact on the immune system. Dendritic cells (DCs) are antigen-presenting cells :APCs) to naive T lymphocytes with a critical role in the regulation of the innate and adaptive immune response. The aim of this work was to study the interaction of monocyte-derived dendritic cells (Mo-DCs) with BVDV and/or BHV-1, as well as the replication and productive infection of these viruses. Monocytes were isolated from PBMCs of heifers seronegative to BVDV and BHV-1 following incubation with CD14-labelled superparamagnetic particles and áifferentiated into Mo-DCs using IL-4 and GM-CSF after incubation for 4 days. Mo-DCs were separated into several groups of infection: Uninfected, infected with noncytopathogenic BVDV-1a, infected with BHV-1.1 and infected with both viruses, being incubated for 24 and 48 hours. We examined both, viral replication and production, and the expression of cell surface markers like MHCI, MHCII, GD80 and CD11b. Results showed that Mo-DCs of the animáis were efficiently infected with BVDV and/or BHV-1.1 in the in vitro conditions tested. Throughout the course of the infection, detection of genetic material from both viruses increased intracellulary and decreased in the culture supernatant; however, the ability of Mo-DCs to produce infectious virus was reduced. In addition, the infection with BHV-1 alone and with both viruses down-regulated the expression of Mo-DCs cell surface markers MHCI, MHCII, CD80 and CD11b, which are critical for DCs function in immune response. These data demónstrate that BHV1 infection of the host APCs may influence the pathogenesis of the infection and hamper the ability to induce an appropriate immune response against an invading pathogen, as BVDV.
|Titolo:||Bovine monocyte-derived dendritic cells in response to infectious BVDV and/or BHV-1.1|
LUZZAGO, CAMILLA (Penultimo)
|Data di pubblicazione:||set-2015|
|Settore Scientifico Disciplinare:||Settore VET/05 - Malattie Infettive degli Animali Domestici|
|Citazione:||Bovine monocyte-derived dendritic cells in response to infectious BVDV and/or BHV-1.1 / M.A. Risalde, F. Romero-Palomo, C. Lecchi, F. Ceciliani, C. Luzzago, J.C. Gómez-Villamandos. ((Intervento presentato al 5. convegno European veterinary immunology workshop tenutosi a Wien nel 2015.|
|Appare nelle tipologie:||14 - Intervento a convegno non pubblicato|