Molecular targeting of the PKC-beta inhibitor enzastaurin (LY317615) in multiple myeloma

An acquired JAK2V617F mutation has been found in myeloid cells from most patients with chronic idiopathic myelofibrosis (IM), but whether it occurs in a common myelo-lymphoid, rather than a myeloid-restricted, progenitor cell is still debated. Using a sensitive ARMS assay for the quantitative assessment of JAK2V617F cDNA, we detected the mutation in purified B, T and NK cells from about half of 12 patients studied. These results indicate that involvement of the lymphoid lineage in IM may be more frequent than previously supposed.