In the study, we assess a rapid prototyped scaffold composed of 30/70 hydroxyapatite (HA) and beta-tricalcium-phosphate (β-TCP) loaded with human adipose-derived stem cells (hASCs) to determine cell proliferation, differentiation toward osteogenic lineage, adhesion and penetration on/into the scaffold.In this in vitro study, hASCs isolated from fat tissue discarded after plastic surgery were expanded, characterized, and then loaded onto the scaffold. Cells were tested for: viability assay (Alamar Blue at days 3, 7 and Live/Dead at day 32), differentiation index (alkaline phosphatase activity at day 14), scaffold adhesion (standard error of the mean analysis at days 5 and 18), and penetration (ground sections at day 32).All the hASC populations displayed stemness markers and the ability to differentiate toward adipogenic and osteogenic lineages.Cellular vitality increased between 3 and 7 days, and no inhibitory effect by HA/β-TCP was observed. Under osteogenic stimuli, scaffold increased alkaline phosphatase activity of +243% compared with undifferentiated samples. Human adipose-derived stem cells adhered on HA/β-TCP surface through citoplasmatic extensions that occupied the macropores and built networks among them. Human adipose derived stem cells were observed in the core of HA/β-TCP. The current combination of hASCs and HA/β-TCP scaffold provided encouraging results. If authors' data will be confirmed in preclinical models, the present engineering approach could represent an interesting tool in treating large bone defects.
Human Adipose-Derived Stem Cells on Rapid Prototyped Three-Dimensional Hydroxyapatite/Beta-Tricalcium Phosphate Scaffold / E. Canciani, C. Dellavia, L.M. Ferreira, C. Giannasi, D. Carmagnola, A. Carrassi, A.T. Brini. - In: THE JOURNAL OF CRANIOFACIAL SURGERY. - ISSN 1049-2275. - 27:3(2016 May), pp. 727-732.
|Titolo:||Human Adipose-Derived Stem Cells on Rapid Prototyped Three-Dimensional Hydroxyapatite/Beta-Tricalcium Phosphate Scaffold|
CANCIANI, ELENA (Primo)
BRINI, ANNA TERESA (Ultimo)
|Parole Chiave:||adipose-derived mesenchymal stem cells; bone regeneration; HA/beta-TCP scaffold; tissue engineering|
|Settore Scientifico Disciplinare:||Settore BIO/14 - Farmacologia|
Settore BIO/13 - Biologia Applicata
|Progetto:||Cellule staminali e scaffold 3D: un biocomplesso innovativo nella rigenerazione ossea|
|Data di pubblicazione:||mag-2016|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1097/SCS.0000000000002567|
|Appare nelle tipologie:||01 - Articolo su periodico|