Infection of Chlorella NC64A cells by PBCV-1 produces a rapid depolarization of the host probably by incorporation of a viral-encoded K+ channel (Kcv) into the host membrane. To examine the effect of an elevated conductance, we monitored the virus-stimulated efflux of K+ from the chlorella cells. The results indicate that all 8 chlorella viruses tested evoked a host specific K+ efflux with a concomitant decrease in the intracellular K+. This K+ efflux is partially reduced by blockers of the Kcv channel. Qualitatively these results support the hypothesis that depolarization and K+ efflux are at least partially mediated by Kcv. The virus-triggered K+ efflux occurs in the same time frame as host cell wall degradation and ejection of viral DNA. Therefore, it is reasonable to postulate that loss of K+ and associated water fluxes from the host lower the pressure barrier to aid ejection of DNA from the virus particles into the host.
|Titolo:||Chlorella viruses evoke a rapid release of K+ from host cells during the early phase of infection|
|Autori interni:||MORONI, ANNA (Penultimo)|
|Parole Chiave:||Chlorella viruses ; viral K+ channel Kcv ; K+ efflux ; Phycodnaviridae ; PBCV-1|
|Settore Scientifico Disciplinare:||Settore BIO/04 - Fisiologia Vegetale|
|Data di pubblicazione:||2008|
|Digital Object Identifier (DOI):||10.1016/j.virol.2007.10.024|
|Appare nelle tipologie:||01 - Articolo su periodico|