The biological implication of protein tyrosine nitration in signaling pathways triggered by nitric oxide is recently emerging. Here we report for the first time that nitrotyrosination occurs in the neural intermediate filament protein peripherin. In neuron-like PC12 cells, nitrated peripherin is associated with the cytoskeleton fraction, its level increases during the progression of NGF-induced differentiation and the nitrated protein remains closely associated with stable microtubules. Tyr 17 and Tyr 376 were identified by MALDI-TOF analyses as two specific residues endogenously nitrated. Finally, peripherin nitration is not restricted to PC12 cells but it is also present in vivo in rat brain.

Tyrosine Nitration is a Novel Post-translational Modification Occurring on the Neuronal Intermediate Filament Protein Peripherin / G. Tedeschi, G. Cappelletti, S. Nonnis, F. Taverna, A. Negri, C. Ronchi, S. Ronchi. - In: NEUROCHEMICAL RESEARCH. - ISSN 0364-3190. - 32:3(2007), pp. 433-441.

Tyrosine Nitration is a Novel Post-translational Modification Occurring on the Neuronal Intermediate Filament Protein Peripherin

G. Tedeschi;G. Cappelletti;S. Nonnis;F. Taverna;A. Negri;C. Ronchi;S. Ronchi
2007

Abstract

The biological implication of protein tyrosine nitration in signaling pathways triggered by nitric oxide is recently emerging. Here we report for the first time that nitrotyrosination occurs in the neural intermediate filament protein peripherin. In neuron-like PC12 cells, nitrated peripherin is associated with the cytoskeleton fraction, its level increases during the progression of NGF-induced differentiation and the nitrated protein remains closely associated with stable microtubules. Tyr 17 and Tyr 376 were identified by MALDI-TOF analyses as two specific residues endogenously nitrated. Finally, peripherin nitration is not restricted to PC12 cells but it is also present in vivo in rat brain.
peripherin; nitric oxide; cytoskeleton; PC12 cells; MALDI-TOF; neuronal differentiation
Settore BIO/10 - Biochimica
Settore BIO/06 - Anatomia Comparata e Citologia
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2434/40556
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