The selective oxidation of D-glucose to D-gluconic acid was performed at atmospheric pressure, controlled pH value, and constant oxygen concentration, in the temperature range from 273.2 to 303.2 K, using Hyderase (a commercial glucose oxidase and catalase preparation). Measurements of initial rate as a function of initial glucose concentration were interpreted by a simplified version of the already proposed reaction mechanism, having the form of a simple equation of the Michaelis–Menten type, with two kinetic parameters, i.e., k1 referring to the reaction between glucose and oxidized enzyme, and kc referring to a combination of the other steps. The activation energy for k1 was found to be 49.6 kJ mol−1; an apparent activation energy of 26.7 kJ mol−1 was obtained for kc.
Aerobic oxidation of glucose. 1 : Enzymatic catalysis / P. Beltrame, M. Comotti, C. Della Pina, M. Rossi. - In: JOURNAL OF CATALYSIS. - ISSN 0021-9517. - 228:2(2004), pp. 282-287.
Aerobic oxidation of glucose. 1 : Enzymatic catalysis
P. BeltramePrimo
;M. ComottiSecondo
;C. Della PinaPenultimo
;M. RossiUltimo
2004
Abstract
The selective oxidation of D-glucose to D-gluconic acid was performed at atmospheric pressure, controlled pH value, and constant oxygen concentration, in the temperature range from 273.2 to 303.2 K, using Hyderase (a commercial glucose oxidase and catalase preparation). Measurements of initial rate as a function of initial glucose concentration were interpreted by a simplified version of the already proposed reaction mechanism, having the form of a simple equation of the Michaelis–Menten type, with two kinetic parameters, i.e., k1 referring to the reaction between glucose and oxidized enzyme, and kc referring to a combination of the other steps. The activation energy for k1 was found to be 49.6 kJ mol−1; an apparent activation energy of 26.7 kJ mol−1 was obtained for kc.Pubblicazioni consigliate
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