The morphofunctional preservation of the blood-brain barrier (BBB) was evaluated in the isolated guinea pig brain maintained in vitro by arterial perfusion. Electron microscopy evaluation after 5 hr in vitro demonstrated that cerebral capillaries and BBB specializations in this preparation retain features compatible with structural integrity. BBB-impermeable and -permeable atropine derivatives arterially perfused to antagonize carbachol-induced fast oscillatory activity confirmed the functional preservation of the BBB in vitro. To study BBB function further, changes in extracellular K+ concentration during arterial perfusion of a high-K+ solution were measured with K+-sensitive electrodes positioned in the cortex and, as control, at the brain venous outlet, where the solution perfused through the brain arterial system was collected. After 5 hr in vitro, the [K+]o values measured during high-K+ perfusion in the piriform and entorhinal cortices were 5.02 ± 0.17 mM (mean ± SE) and 5.2 ± 0.21 mM, respectively (n = 6). Coperfusion of the high-K+ solution with the Na+/K+ pump blocker ouabain (10 M; n = 4) induced consistently spreading depression preceded by a rise in [K+]o. Finally, sporadic, isolated spots of extravasation of the fluorescent marker fluorescein isothiocyanate (FITC)-dextran preferentially circumscribed to deep cortical layers was observed in brains perfused with FITC-dextran after 5 hr in vitro. The study demonstrates that the in vitro isolated guinea pig brain is viable for studying cerebrovascular interactions and BBB permeability of compounds active in the central nervous system.
|Titolo:||Blood-brain barrier preservation in the in vitro isolated guinea pig brain preparation|
DE BIASI, SILVIA (Penultimo)
|Parole Chiave:||Cerebrovascular interactions; Cortex; Endothelial cells; Isolated in vitro brain; Potassium|
|Settore Scientifico Disciplinare:||Settore BIO/06 - Anatomia Comparata e Citologia|
|Data di pubblicazione:||2001|
|Digital Object Identifier (DOI):||10.1002/jnr.1223|
|Appare nelle tipologie:||01 - Articolo su periodico|