We investigated the intracellular distribution of heat shock factors 1 and 2 (HSF1, HSF2) in rat spinal cord by immunoblotting and immunohistochemistry using selective policlonal antibodies. Our results showed that both HSF1 and HSF2 were expressed in spinal cord cells (both neurons and glia) but at different intensity and cell localization. HSF1 was unusually distributed in the perinuclear compartment of selected neurons of the gray matter while astrocytes, oligodendrocytes and ependymal cells were predominantly stained in the nucleus. HSF2 was expressed at lower levels than HSF1 and was scattered in both nucleus and cytoplasm of the motoneurons of the ventral horns while glial cells again showed a nuclear positivity. This study suggested that the different ability of neurons vs. glial cells to react against adverse conditions might well be correlated with the different constitutive localization of HSFs.

Cell-specific expression of heat shock transcription factors 1 and 2 in unstressed rat spinal cord / A. Stacchiotti, R. Rezzani, L. Rodella, L. Tiberio, L. Schiaffonati, R. Bianchi. - In: NEUROSCIENCE LETTERS. - ISSN 0304-3940. - 268:2(1999 Jun 18), pp. 73-76. [10.1016/S0304-3940(99)00394-8]

Cell-specific expression of heat shock transcription factors 1 and 2 in unstressed rat spinal cord

A. Stacchiotti;R. Bianchi
Ultimo
1999

Abstract

We investigated the intracellular distribution of heat shock factors 1 and 2 (HSF1, HSF2) in rat spinal cord by immunoblotting and immunohistochemistry using selective policlonal antibodies. Our results showed that both HSF1 and HSF2 were expressed in spinal cord cells (both neurons and glia) but at different intensity and cell localization. HSF1 was unusually distributed in the perinuclear compartment of selected neurons of the gray matter while astrocytes, oligodendrocytes and ependymal cells were predominantly stained in the nucleus. HSF2 was expressed at lower levels than HSF1 and was scattered in both nucleus and cytoplasm of the motoneurons of the ventral horns while glial cells again showed a nuclear positivity. This study suggested that the different ability of neurons vs. glial cells to react against adverse conditions might well be correlated with the different constitutive localization of HSFs.
Heat shock proteins; Heat shock transcription factors; Immunocytochemistry; Rat; Spinal cord
18-giu-1999
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/39084
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