High-performance liquid chromatography (HPLC) is still, today, the technique of choice for the analysis of natural dyes in artistic objects and historical textiles, particularly in association with photodiode array (PDA) detection. In the last two decades, surfaceenhanced Raman spectroscopy (SERS) gained also increasing importance for these investigations thanks to its sensitivity and limited requirements in terms of sample quantity. In favor of SERS, its high specificity inmolecular recognition typical of vibrational spectroscopy should be mentioned, whereas this non-separative technique is obviously disadvantaged in the analyses of mixed chromophores, as is often the case of many natural dyes and also of tints obtained by the combined use of different colorants. An optimized experimental setup combining the two techniques, HPLC-PDA and SERS, is proposed in the present work, allowing online SERS detection of different dyeing compounds eluted from the HPLC column. Examples are presented concerning some of the colorants most widely used in history, such as morin and luteolin for yellow dyes, alizarin, purpurin, laccaic, kermesic, and carminic acids for red ones, and indigotin for blue tints.
Online coupling of high-performance liquid chromatography with surface-enhanced Raman spectroscopy for the identification of historical dyes / C. Zaffino, G.D. Bedini, G. Mazzola, V. Guglielmi, S. Bruni. - In: JOURNAL OF RAMAN SPECTROSCOPY. - ISSN 0377-0486. - 47:5(2016 May), pp. 607-615.
Online coupling of high-performance liquid chromatography with surface-enhanced Raman spectroscopy for the identification of historical dyes
C. ZaffinoPrimo
;V. GuglielmiPenultimo
;S. Bruni
2016
Abstract
High-performance liquid chromatography (HPLC) is still, today, the technique of choice for the analysis of natural dyes in artistic objects and historical textiles, particularly in association with photodiode array (PDA) detection. In the last two decades, surfaceenhanced Raman spectroscopy (SERS) gained also increasing importance for these investigations thanks to its sensitivity and limited requirements in terms of sample quantity. In favor of SERS, its high specificity inmolecular recognition typical of vibrational spectroscopy should be mentioned, whereas this non-separative technique is obviously disadvantaged in the analyses of mixed chromophores, as is often the case of many natural dyes and also of tints obtained by the combined use of different colorants. An optimized experimental setup combining the two techniques, HPLC-PDA and SERS, is proposed in the present work, allowing online SERS detection of different dyeing compounds eluted from the HPLC column. Examples are presented concerning some of the colorants most widely used in history, such as morin and luteolin for yellow dyes, alizarin, purpurin, laccaic, kermesic, and carminic acids for red ones, and indigotin for blue tints.File | Dimensione | Formato | |
---|---|---|---|
Zaffino et al_Journal of Raman Spectroscopy 2016 (2).pdf
accesso riservato
Tipologia:
Publisher's version/PDF
Dimensione
1.43 MB
Formato
Adobe PDF
|
1.43 MB | Adobe PDF | Visualizza/Apri Richiedi una copia |
Pubblicazioni consigliate
I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.