This study presents a custom-made in situ gelling polymeric precursor for cell encapsulation. Composed of poly((2-hydroxyethyl)methacrylate-co-(3-aminopropyl)methacrylamide) (P(HEMA-co-APM) mother backbone and RGD-mimicking poly(amidoamine) (PAA) moiteis, the comb-like structured polymeric precursor is tailored to gather the advantages of the two families of synthetic polymers, i.e., the good mechanical integrity of PHEMA-based polymers and the biocompatibility and biodegradability of PAAs. The role of P(HEMA-co-APM) in the regulation of the chemico-physical properties of P(HEMA-co-APM)/PAA hydrogels is thoroughly investigated. On the basis of obtained results, namely the capability of maintaining vital NIH3T3 cell line in vitro for 2 d in a 3D cell culture, the in vivo biocompatibility in murine model for 16 d, and the ability of finely tuning mechanical properties and degradation kinetics, it can be assessed that P(HEMA-co-APM)/PAAs offer a cost-effective valid alternative to the so far studied natural polymer-based systems for cell encapsulation.
A Tailor-Made Synthetic Polymer for Cell Encapsulation : Design Rationale, Synthesis, Chemical-Physics and Biological Characterizations / I. Gerges, M. Tamplenizza, E. Rossi, A. Tocchio, F. Martello, C. Recordati, D. Kumar, N.R. Forsyth, Y. Liu, C. Lenardi. - In: MACROMOLECULAR BIOSCIENCE. - ISSN 1616-5187. - 16:6(2016 Jun), pp. 870-881.
Titolo: | A Tailor-Made Synthetic Polymer for Cell Encapsulation : Design Rationale, Synthesis, Chemical-Physics and Biological Characterizations | |
Autori: | GERGES, IRINI (Primo) TAMPLENIZZA, MARGHERITA (Secondo) LENARDI, CRISTINA (Ultimo) | |
Parole Chiave: | cell encapsulation; comb-like polymer; degradation kinetics; mechanical properties; photo-cross-linking; sol-gel; biotechnology; bioengineering; biomaterials; polymers and plastics; materials chemistry2506 metals and alloys | |
Settore Scientifico Disciplinare: | Settore FIS/07 - Fisica Applicata(Beni Culturali, Ambientali, Biol.e Medicin) | |
Data di pubblicazione: | giu-2016 | |
Rivista: | ||
Tipologia: | Article (author) | |
Digital Object Identifier (DOI): | http://dx.doi.org/10.1002/mabi.201500386 | |
Appare nelle tipologie: | 01 - Articolo su periodico |