Nicotine is the primary addictive substance in tobacco smoke and electronic cigarette (e-cig) vapour. Methodological limitations have made it difficult to compare the role of the nicotine and non-nicotine constituents of tobacco smoke. The aim of this study was to compare the effects of traditional cigarette smoke and e-cig vapour containing the same amount of nicotine in male BALB/c mice exposed to the smoke of 21 cigarettes or e-cig vapour containing 16.8. mg of nicotine delivered by means of a mechanical ventilator for three 30-min sessions/day for seven weeks. One hour after the last session, half of the animals were sacrificed for neurochemical analysis, and the others underwent mecamylamine-precipitated or spontaneous withdrawal for the purposes of behavioural analysis. Chronic intermittent non-contingent, second-hand exposure to cigarette smoke or e-cig vapour led to similar brain cotinine and nicotine levels, similar urine cotinine levels and the similar up-regulation of α4β2 nicotinic acetylcholine receptors in different brain areas, but had different effects on body weight, food intake, and the signs of mecamylamine-precipitated and spontaneous withdrawal episodic memory and emotional responses. The findings of this study demonstrate for the first time that e-cig vapour induces addiction-related neurochemical, physiological and behavioural alterations. The fact that inhaled cigarette smoke and e-cig vapour have partially different dependence-related effects indicates that compounds other than nicotine contribute to tobacco dependence.
Different physiological and behavioural effects of e-cigarette vapour and cigarette smoke in mice / L. Ponzoni, M. Moretti, M. Sala, F. Fasoli, V. Mucchietto, V. Lucini, G. Cannazza, G. Gallesi, C.N. Castellana, F. Clementi, M. Zoli, C. Gotti, D. Braida. - In: EUROPEAN NEUROPSYCHOPHARMACOLOGY. - ISSN 0924-977X. - 25:10(2015), pp. 1775-1786.
|Titolo:||Different physiological and behavioural effects of e-cigarette vapour and cigarette smoke in mice|
PONZONI, LUISA (Primo)
MORETTI, MILENA (Secondo)
|Parole Chiave:||Nicotine; Nicotinic receptors; Cigarette smoke; Electronic cigarettes, mecamylamine precipitated withdrawal; Spontaneous withdrawal|
|Settore Scientifico Disciplinare:||Settore BIO/14 - Farmacologia|
|Data di pubblicazione:||2015|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1016/j.euroneuro.2015.06.010|
|Appare nelle tipologie:||01 - Articolo su periodico|