The pore forming activity of Cry1Ab, Cry1Fa and Cry1Ca toxins and their interaction with leucine transport mediated by the K+/leucine cotransporter were studied in brush border membrane vesicles (BBMV) isolated from the midgut of Ostrinia nubilalis and Sesamia nonagrioides. In both species, as in other Lepidoptera, leucine uptake by BBMV can take place in the absence of cations but it can also be driven by a K+- gradient. Experiments with the voltage-sensitive fluorescent dye DiSC3(5) proved that Cry1Ab, a Bt toxin active in vivo, enhanced the membrane permeability to potassium in O. nubilalis-BBMV. This result is in agreement with similar effects observed in S. nonagrioides-BBMV incubated with various Cry1 toxins active in vivo. The effect of the above toxins was tested on the initial rate of 0.1 mM leucine influx. Instead of an increase in leucine influx, a reduction was observed with the Cry1 toxins active in vivo. Cry1Ab and Cry1Fa, but not the inactive toxin Cry1Da, inhibited in a dose-dependent manner leucine uptake both in the absence and in the presence of a K+-gradient, a clear indication that their effect is independent of the channel formed by the toxins and that this effect is exerted directly on the amino acid transport system.
Leucine transport is affected by Bacillus thuringiensis Cry1 toxins in brush border membrane vesicles from Ostrinia nubilalis Hb (Lepidoptera: Pyralidae) and Sesamia nonagrioides Lefebvre (Lepidoptera: Noctuidae) midgut / M.G. Leonardi, S. Caccia, J. González-Cabrera, J. Ferré, B.C. Giordana. - In: THE JOURNAL OF MEMBRANE BIOLOGY. - ISSN 0022-2631. - 214:3(2006), pp. 157-164.
Leucine transport is affected by Bacillus thuringiensis Cry1 toxins in brush border membrane vesicles from Ostrinia nubilalis Hb (Lepidoptera: Pyralidae) and Sesamia nonagrioides Lefebvre (Lepidoptera: Noctuidae) midgut
M.G. Leonardi;S. Caccia;B.C. Giordana
2006
Abstract
The pore forming activity of Cry1Ab, Cry1Fa and Cry1Ca toxins and their interaction with leucine transport mediated by the K+/leucine cotransporter were studied in brush border membrane vesicles (BBMV) isolated from the midgut of Ostrinia nubilalis and Sesamia nonagrioides. In both species, as in other Lepidoptera, leucine uptake by BBMV can take place in the absence of cations but it can also be driven by a K+- gradient. Experiments with the voltage-sensitive fluorescent dye DiSC3(5) proved that Cry1Ab, a Bt toxin active in vivo, enhanced the membrane permeability to potassium in O. nubilalis-BBMV. This result is in agreement with similar effects observed in S. nonagrioides-BBMV incubated with various Cry1 toxins active in vivo. The effect of the above toxins was tested on the initial rate of 0.1 mM leucine influx. Instead of an increase in leucine influx, a reduction was observed with the Cry1 toxins active in vivo. Cry1Ab and Cry1Fa, but not the inactive toxin Cry1Da, inhibited in a dose-dependent manner leucine uptake both in the absence and in the presence of a K+-gradient, a clear indication that their effect is independent of the channel formed by the toxins and that this effect is exerted directly on the amino acid transport system.Pubblicazioni consigliate
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