The genetic heterogeneity of hepatitis B virus (HBV) genotypes and subgenotypes was investigated by directly sequencing amplified PreS, S and P genes of HBV isolates obtained from the plasma of 99 subjects with chronic HBV infection. Genotype D showed the greatest intragenotypic and intrasubgenotypic divergence: in particular, the a determinant was mutated in 58.2% of the genotype D patients, two of whom showed prototypic vaccine-induced escape mutants at codon 145. Moreover, five sites under significant positive selection were found in the S protein of the D isolates: one in the a determinant and four in the highly hydrophobic C terminal. Our results suggest that careful surveillance of vaccine-induced escape mutants should be considered in populations with highly frequent genotype D infections, and raise questions concerning the possible relationship between the genetic heterogeneity, host immunity and pathogenicity of this HBV genotype.
High level of genetic heterogeneity in S and P genes of genotype D hepatitis B virus / C. De Maddalena, C. Giambelli, E. Tanzi, D. Colzani, M. Schiavini, L. Milazzo, F. Bernini, E. Ebranati, A. Cargnel, R. Bruno, M. Galli, G. Zehender. - In: VIROLOGY. - ISSN 0042-6822. - 365:1(2007 Aug 15), pp. 113-124.
|Titolo:||High level of genetic heterogeneity in S and P genes of genotype D hepatitis B virus|
DE MADDALENA, CHIARA (Primo)
GIAMBELLI, CAMILLA (Secondo)
GALLI, MASSIMO (Penultimo)
ZEHENDER, GIANGUGLIELMO (Ultimo)
|Parole Chiave:||Genetic heterogeneity; Genetic variability; Genotypes; Hepatitis B virus; Molecular evolution; Natural selection; Pathogenesis|
|Settore Scientifico Disciplinare:||Settore MED/42 - Igiene Generale e Applicata|
Settore MED/17 - Malattie Infettive
|Data di pubblicazione:||15-ago-2007|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1016/j.virol.2007.03.015|
|Appare nelle tipologie:||01 - Articolo su periodico|