The degradative properties towards chlorobenzoates by a 2-chlorobenzoate-degrading mixed culture (2MC) were investigated. Although 2MC did not grow on 2,3,5-; 2,3,6- and 2,4,6-trichlorobenzoates, it was able to completely oxidise 2,3,5-trichlorobenzoate and 2,5-dichlorobenzoate. This was degraded through the intermediate formation of 4-chlorocatechol and 3-cis,cis-chloromuconate, suggesting a dioxygenation in 1,2 position. 4-Chlorobenzoate; 3,4- and 2,6-dichlorobenzoate; 2,4,6- and 2,3,6-trichlorobenzoate did not undergo any co-metabolic transformation, the only 2,4-dichlorobenzoate was scarcely transformed into a dichlorinated phenol as end-product. 2MC was constituted of three prominent species: Stenotrophomonas maltophilia, Cupriavidus necator and Flavobacterium sp. Ring-hydroxylating dioxygenase genes (BenA) were retrieved in S. maltophilia and C. necator, indicating the presence of a “modified ortho pathway” in the culture. On the basis of its degradative properties, 2MC could be a good candidate for use in the removal of ortho- and/or meta-chlorobenzoates that are the major end-products of PCB co-metabolism.

Co-metabolism of di- and trichlorobenzoates in a 2-chlorobenzoate-degrading bacterial culture : Effect of the position and number of halo-substituents / G. Baggi, S. Bernasconi, M.E. Zangrossi, L. Cavalca, V. Andreoni. - In: INTERNATIONAL BIODETERIORATION & BIODEGRADATION. - ISSN 0964-8305. - 62:1(2008), pp. 57-64.

Co-metabolism of di- and trichlorobenzoates in a 2-chlorobenzoate-degrading bacterial culture : Effect of the position and number of halo-substituents

G. Baggi
Primo
;
S. Bernasconi
Secondo
;
M.E. Zangrossi;L. Cavalca
Penultimo
;
V. Andreoni
Ultimo
2008

Abstract

The degradative properties towards chlorobenzoates by a 2-chlorobenzoate-degrading mixed culture (2MC) were investigated. Although 2MC did not grow on 2,3,5-; 2,3,6- and 2,4,6-trichlorobenzoates, it was able to completely oxidise 2,3,5-trichlorobenzoate and 2,5-dichlorobenzoate. This was degraded through the intermediate formation of 4-chlorocatechol and 3-cis,cis-chloromuconate, suggesting a dioxygenation in 1,2 position. 4-Chlorobenzoate; 3,4- and 2,6-dichlorobenzoate; 2,4,6- and 2,3,6-trichlorobenzoate did not undergo any co-metabolic transformation, the only 2,4-dichlorobenzoate was scarcely transformed into a dichlorinated phenol as end-product. 2MC was constituted of three prominent species: Stenotrophomonas maltophilia, Cupriavidus necator and Flavobacterium sp. Ring-hydroxylating dioxygenase genes (BenA) were retrieved in S. maltophilia and C. necator, indicating the presence of a “modified ortho pathway” in the culture. On the basis of its degradative properties, 2MC could be a good candidate for use in the removal of ortho- and/or meta-chlorobenzoates that are the major end-products of PCB co-metabolism.
(Chloro)benzoate dioxygenase; Biodegradation; Co-metabolism; Halo-substituent; meta-; Ortho-; para-position
Settore AGR/16 - Microbiologia Agraria
Settore CHIM/06 - Chimica Organica
2008
Article (author)
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/37690
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