The analysis of mesenchymal stromal cells secretome is fundamental to identify key players of processes involving these cells. Truly secreted proteins may be difficult to detect in MS based analysis of conditioned media (CM) due to proteins supplemented with fetal bovine serum (FBS). We compared different growth conditions to determine the effect of varying FBS concentration on the number and quantity of truly secreted human proteins vs contaminating bovine proteins. The results suggest that to minimize interference cells should be grown in presence of FBS until confluence and transferred into a serum-free medium prior to secretome collection.

Effect of fetal bovine serum in culture media on MS analysis of mesenchymal stromal cells secretome / S. Nonnis, E. Maffioli, L. Zanotti, F. Santagata, A. Negri, A. Viola, S. Elliman, G. Tedeschi. - In: EUPA OPEN PROTEOMICS. - ISSN 2212-9685. - 10:(2016), pp. 28-30. [10.1016/j.euprot.2016.01.005]

Effect of fetal bovine serum in culture media on MS analysis of mesenchymal stromal cells secretome

S. Nonnis
;
E. Maffioli;F. Santagata;A. Negri;G. Tedeschi
Ultimo
2016

Abstract

The analysis of mesenchymal stromal cells secretome is fundamental to identify key players of processes involving these cells. Truly secreted proteins may be difficult to detect in MS based analysis of conditioned media (CM) due to proteins supplemented with fetal bovine serum (FBS). We compared different growth conditions to determine the effect of varying FBS concentration on the number and quantity of truly secreted human proteins vs contaminating bovine proteins. The results suggest that to minimize interference cells should be grown in presence of FBS until confluence and transferred into a serum-free medium prior to secretome collection.
Fetal bovine serum; Mesenchymal stem cells; Secretome; Tandem mass spectrometry; Biochemistry
Settore BIO/10 - Biochimica
2016
Article (author)
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/376617
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