In this work we evaluated the combined use of AFLP markers and selective genotyping in a Grand Daughter experimental design (GDD), to identify chromosomal regions candidate to contain QTL for milk protein percentage (PP) in Italian Friesian cattle. We verified the approach by microsatellite analysis of 4 candidate chromosomal regions. Twenty-five AFLP primer combinations, assayed on the extreme minus and plus-variant half-sibs and on the grandsires of 2 GDD families, produced 180 polymorphic bands of paternal origin. Association analysis resulted in 16 AFLPs significantly associated to PP-EBVs (Pless than or equal to 0.05), that mapped on 8 chromosomes, in regions where PP-QTLs were identified in previous studies. Microsatellite analysis confirmed association for 2 regions of BTA10 and BTA28

Identification of milk protein percentage QTLs in Italian Friesian cattle by selective genotyping two GDD families with AFLP and SSR markers / P. Ajmone Marsan, E. Milanesi, F. Schiavini, R. Mazza, R. Negrini. - In: ITALIAN JOURNAL OF ANIMAL SCIENCE. - ISSN 1594-4077. - 6:Suppl.1(2007), pp. 40-42. [10.4081/ijas.2007.1s.272]

Identification of milk protein percentage QTLs in Italian Friesian cattle by selective genotyping two GDD families with AFLP and SSR markers

E. Milanesi
Secondo
;
F. Schiavini;
2007

Abstract

In this work we evaluated the combined use of AFLP markers and selective genotyping in a Grand Daughter experimental design (GDD), to identify chromosomal regions candidate to contain QTL for milk protein percentage (PP) in Italian Friesian cattle. We verified the approach by microsatellite analysis of 4 candidate chromosomal regions. Twenty-five AFLP primer combinations, assayed on the extreme minus and plus-variant half-sibs and on the grandsires of 2 GDD families, produced 180 polymorphic bands of paternal origin. Association analysis resulted in 16 AFLPs significantly associated to PP-EBVs (Pless than or equal to 0.05), that mapped on 8 chromosomes, in regions where PP-QTLs were identified in previous studies. Microsatellite analysis confirmed association for 2 regions of BTA10 and BTA28
Settore AGR/17 - Zootecnica Generale e Miglioramento Genetico
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2434/37461
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