OBJECTIVE: To compare directly in vitro developmental competence between parthenogenetically activated and intracytoplasmic sperm injection (ICSI)-fertilized oocytes. DESIGN: For each patient, three metaphase II oocytes were randomized to the ICSI procedure, while n-3 were allocated to parthenogenetic activation. SETTING: University hospital infertility unit. PATIENTS: Thirty-eight patients, aged 35.2 +/- 3.3 years (mean +/- SD) selected for ICSI. INTERVENTIONS: After 1 hour from denudation, oocytes were either fertilized by ICSI (n = 114) or chemically activated (n = 104). Fertilized and activated oocytes were cultured for up to 3 and 5 days, respectively. MAIN OUTCOME MEASURES: Development rate, cell number, and morphological grade during culture. RESULTS: The two groups showed no significant differences between rates of fertilization and parthenogenetic activation, development, and blastomere number on days 2 and 3 of culture. However, parthenotes showed a lower morphological grade, and a significantly lower proportion went on cleaving to day 3, when only activated rather than total numbers of oocytes were considered. On day 5 after activation, nine oocytes (8.6%) reached the blastocyst stage, representing 12.9% of parthenotes. CONCLUSIONS: Since most parameters examined in this study were similar between activated and fertilized oocytes, parthenogenetic activation may be a useful tool for the preclinical evaluation of experimental procedures.
|Titolo:||In vitro development of human oocytes after parthenogenetic activation or intracytoplasmic sperm injection|
|Parole Chiave:||blastocyst; ICSI; in vitro development; Parthenogenesis|
|Settore Scientifico Disciplinare:||Settore VET/01 - Anatomia degli Animali Domestici|
|Data di pubblicazione:||2007|
|Digital Object Identifier (DOI):||10.1016/j.fertnstert.2006.05.063|
|Appare nelle tipologie:||01 - Articolo su periodico|