The Pseudomonas aeruginosa PA3685 locus encodes a conserved protein that shares 49% sequence identity with Escherichia coli YeaZ, which was recently reported as involved in the biosynthesis of threonylcarbamoyl adenosine (t6A), a universal modified tRNA nucleoside. Many YeaZ orthologues were reported as "essential for life" among various bacterial species, suggesting a critical role for both these proteins and for the t6A biosynthetic pathway. We provide here evidences that PA3685 protein (PaYeaZ) is essential. Additionally, we describe its purification, crystallization, and crystallographic structure. The crystal structure shows that PaYeaZ is composed of two domains one of which is the platform to form protein-protein interaction involved either in homodimeric assembly or in the formation of the multiprotein complex required for the synthesis of t6A. These features make the PaYeaZ protein a potential target candidate for the design of novel inhibitors able to hinder the complex formation and expected to abolish the crucial activity of t6A synthesis.
Crystal structure of YeaZ from Pseudomonas aeruginosa / D. Vecchietti, S. Ferrara, R. Rusmini, R. Macchi, M. Milani, G. Bertoni. - In: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS. - ISSN 0006-291X. - 470:2(2016), pp. 460-465.
|Titolo:||Crystal structure of YeaZ from Pseudomonas aeruginosa|
VECCHIETTI, DAVIDE GIOVANNI (Primo)
FERRARA, SILVIA (Secondo)
BERTONI, GIOVANNI (Ultimo)
|Parole Chiave:||Crystal structure; Essential gene; Protein characterization; Pseudomonas aeruginosa; Threonylcarbamoyl adenosine synthesis; Biochemistry; Biophysics; Cell Biology; Molecular Biology|
|Settore Scientifico Disciplinare:||Settore BIO/10 - Biochimica|
|Data di pubblicazione:||2016|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1016/j.bbrc.2016.01.008|
|Appare nelle tipologie:||01 - Articolo su periodico|