OBJECTIVES: The quality of whole blood (WB) units is influenced by many factors, starting with selection of donors and the method of blood collection The aim of this study was to investigate the changes that occur in haematological and selected biochemical parameters in blood transferred from a feline blood donor to feline WB unit. METHODS: Data from 27 feline blood donations were used in this study. Cats were anaesthetised with a combination of tiletamine and zolazepam. Blood (10 ml/kg body weight to a maximum of 60 ml/cat) was collected in citrate-phosphate-dextrose-adenine (CPDA) anticoagulant. Lactated Ringer's solution (10 ml/kg) was administered intravenously starting halfway through the donation. Haematological and selected biochemical parameters (complete blood count, free haemoglobin, % haemolysis, glucose, sodium, potassium, pH) were measured in the blood donor before donation and in the corresponding donated WB unit soon after collection. RESULTS: Significant decreases occurred between blood donor and WB unit in red blood cells (mean difference -1.06 × 1012/l; P <0.0001), haemoglobin (mean difference -1.6 g/dl; P <0.0001), haematocrit (mean difference -4.6%; P <0.0001), red cell distribution width (mean difference -0.9%; P = 0.0003), white blood cells (mean difference -2.17 × 109/l; P <0.0001), pH (mean difference -0.5; P <0.0001) and potassium (mean difference -1.4 mmol/l; P <0.0001). Significant increases occurred between blood donor and WB unit in platelets (mean difference +87.00 ×109/l; P = 0.0039), glucose (mean difference +25.42 mmol/l; P <0.0001) and sodium (mean difference +20 mmol/l; P <0.0001). CONCLUSIONS AND RELEVANCE: When using a blood collection protocol with intravenous fluid administration midway through the donation and a CPDA:blood ratio of 1:7, there were significant changes in both the haematological and biochemical characteristics between the blood donors and WB units. The majority of these changes may be the result of the anticoagulants used for storage. Understanding these changes may assist selection of blood donors and help prediction of the characteristics of the donated WB unit.

Change in haematological and selected biochemical parameters measured in feline blood donors and feline whole blood donated units / E. Spada, D. Proverbio, L. Baggiani, G. Bagnagatti De Giorgi, E. Ferro, R. Perego. - In: JOURNAL OF FELINE MEDICINE AND SURGERY. - ISSN 1098-612X. - 19:4(2017 Apr), pp. 375-381. [10.1177/1098612X16628919]

Change in haematological and selected biochemical parameters measured in feline blood donors and feline whole blood donated units

E. Spada
Primo
Conceptualization
;
D. Proverbio
Secondo
;
L. Baggiani;G. Bagnagatti De Giorgi;E. Ferro
Penultimo
;
R. Perego
Ultimo
2017-04

Abstract

OBJECTIVES: The quality of whole blood (WB) units is influenced by many factors, starting with selection of donors and the method of blood collection The aim of this study was to investigate the changes that occur in haematological and selected biochemical parameters in blood transferred from a feline blood donor to feline WB unit. METHODS: Data from 27 feline blood donations were used in this study. Cats were anaesthetised with a combination of tiletamine and zolazepam. Blood (10 ml/kg body weight to a maximum of 60 ml/cat) was collected in citrate-phosphate-dextrose-adenine (CPDA) anticoagulant. Lactated Ringer's solution (10 ml/kg) was administered intravenously starting halfway through the donation. Haematological and selected biochemical parameters (complete blood count, free haemoglobin, % haemolysis, glucose, sodium, potassium, pH) were measured in the blood donor before donation and in the corresponding donated WB unit soon after collection. RESULTS: Significant decreases occurred between blood donor and WB unit in red blood cells (mean difference -1.06 × 1012/l; P <0.0001), haemoglobin (mean difference -1.6 g/dl; P <0.0001), haematocrit (mean difference -4.6%; P <0.0001), red cell distribution width (mean difference -0.9%; P = 0.0003), white blood cells (mean difference -2.17 × 109/l; P <0.0001), pH (mean difference -0.5; P <0.0001) and potassium (mean difference -1.4 mmol/l; P <0.0001). Significant increases occurred between blood donor and WB unit in platelets (mean difference +87.00 ×109/l; P = 0.0039), glucose (mean difference +25.42 mmol/l; P <0.0001) and sodium (mean difference +20 mmol/l; P <0.0001). CONCLUSIONS AND RELEVANCE: When using a blood collection protocol with intravenous fluid administration midway through the donation and a CPDA:blood ratio of 1:7, there were significant changes in both the haematological and biochemical characteristics between the blood donors and WB units. The majority of these changes may be the result of the anticoagulants used for storage. Understanding these changes may assist selection of blood donors and help prediction of the characteristics of the donated WB unit.
Settore VET/08 - Clinica Medica Veterinaria
3-feb-2016
Article (author)
File in questo prodotto:
File Dimensione Formato  
Journal of Feline Medicine and Surgery-2016-Spada-1098612X16628919-1.pdf

accesso riservato

Tipologia: Publisher's version/PDF
Dimensione 790 kB
Formato Adobe PDF
790 kB Adobe PDF   Visualizza/Apri   Richiedi una copia
Change JFMS.pdf

accesso aperto

Tipologia: Post-print, accepted manuscript ecc. (versione accettata dall'editore)
Dimensione 308.06 kB
Formato Adobe PDF
308.06 kB Adobe PDF Visualizza/Apri
1098612x16628919.pdf

accesso riservato

Tipologia: Publisher's version/PDF
Dimensione 94.97 kB
Formato Adobe PDF
94.97 kB Adobe PDF   Visualizza/Apri   Richiedi una copia
Pubblicazioni consigliate

Caricamento pubblicazioni consigliate

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2434/362865
Citazioni
  • ???jsp.display-item.citation.pmc??? 2
  • Scopus 7
  • ???jsp.display-item.citation.isi??? 7
social impact