Seminolipids 1a and 1b and galactosylalkylacylglycerols 2a and 2b, labelled with deuterium on the alkyl or acyl chain, respectively, were obtained isotopically and chemically pure through a straightforward synthesis from protected glycidyl galactoside 3 in an overall 22% yield. The identity and purity of compounds was ascertained by NMR spectroscopy and ESI mass spectrometry analysis. These labelled compounds are important as internal standards for quantification of these lipids by mass spectrometry, and they could also be used in metabolic studies in in vitro and even in vivo systems. Extension of the procedure could provide a route for the preparation of isotopomers of other compounds of the same general class.
An efficient and convenient synthesis of deuterium-labelled seminolipid isotopomers and their ESI-MS characterization / L. Franchini, L. Panza, K. Kongmanas, N. Tanphaichitr, K.F. Faull, F. Ronchetti. - In: CHEMISTRY AND PHYSICS OF LIPIDS. - ISSN 0009-3084. - 152:2(2008 Apr), pp. 78-85.
|Titolo:||An efficient and convenient synthesis of deuterium-labelled seminolipid isotopomers and their ESI-MS characterization|
FRANCHINI, LAURA (Primo)
RONCHETTI, FIAMMA CLELIA VIRGINIA (Ultimo)
|Parole Chiave:||Deuterium isotopomers; ESI-MS; Mammalian sulfoglycolipids; Seminolipid; Sperm-egg interaction|
|Settore Scientifico Disciplinare:||Settore BIO/10 - Biochimica|
|Data di pubblicazione:||apr-2008|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1016/j.chemphyslip.2008.02.002|
|Appare nelle tipologie:||01 - Articolo su periodico|