Naturally occurring CD4(+)CD25(+) T regulatory cells (nTregs) play a key role as suppressors in immune mechanisms that protect against self-destruction. The forkhead box p3 transcription factor (FOXP3) has a central role in the development of nTregs. We show here that co-culture of naïve T cells with flagellin-exposed monocyte-derived dendritic cells (MoDCs) generates CD4(+)CD25(+)FOXP3(+) T cells that transiently express FOXP3 together with CD25 but do not suppress proliferation of CD4(+)CD25(-) T cells. Moreover, purified CD4(+)CD25(+)FOXP3(+) T cells reveal a different proliferation and cytokine production profile from that of nTregs. These data indicate that in the presence of ongoing immune responses a T cell antigenic phenotype superimposable of that of nTregs does not necessarily predict suppressive function and that FOXP3 in humans is not sufficient for development and function of regulatory T cells.

Matured human monocyte-derived dendritic cells (MoDCs) induce expansion of CD4(+)CD25(+) FOXP3(+) T cells lacking regulatory properties / A. Merlo, E. Tagliabue, S. Menard, A. Balsari. - In: IMMUNOLOGY LETTERS. - ISSN 0165-2478. - 117:1(2008 Apr), pp. 106-113. [10.1016/j.imlet.2008.01.006]

Matured human monocyte-derived dendritic cells (MoDCs) induce expansion of CD4(+)CD25(+) FOXP3(+) T cells lacking regulatory properties

A. Balsari
Ultimo
2008

Abstract

Naturally occurring CD4(+)CD25(+) T regulatory cells (nTregs) play a key role as suppressors in immune mechanisms that protect against self-destruction. The forkhead box p3 transcription factor (FOXP3) has a central role in the development of nTregs. We show here that co-culture of naïve T cells with flagellin-exposed monocyte-derived dendritic cells (MoDCs) generates CD4(+)CD25(+)FOXP3(+) T cells that transiently express FOXP3 together with CD25 but do not suppress proliferation of CD4(+)CD25(-) T cells. Moreover, purified CD4(+)CD25(+)FOXP3(+) T cells reveal a different proliferation and cytokine production profile from that of nTregs. These data indicate that in the presence of ongoing immune responses a T cell antigenic phenotype superimposable of that of nTregs does not necessarily predict suppressive function and that FOXP3 in humans is not sufficient for development and function of regulatory T cells.
Settore MED/04 - Patologia Generale
apr-2008
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/35438
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