The epigenetic mechanisms that enable lifelong neurogenesis from neural stem cells (NSCs) in the adult mammalian brain are poorly understood. Here, we show that JMJD3, a histone H3 lysine 27 (H3K27) demethylase, acts as a critical activator of neurogenesis from adult subventricular zone (SVZ) NSCs. JMJD3 is upregulated in neuroblasts, and Jmjd3 deletion targeted to SVZ NSCs in both developing and adult mice impairs neuronal differentiation. JMJD3 regulates neurogenic gene expression via interaction at not only promoter regions but also neurogenic enhancer elements. JMJD3 localizes at neural enhancers genome-wide in embryonic brain, and in SVZ NSCs, JMJD3 regulates the I12b enhancer of Dlx2. In Jmjd3-deleted SVZ cells, I12b remains enriched with H3K27me3 and Dlx2-dependent neurogenesis fails. These findings support a model in which JMJD3 and the poised state of key transcriptional regulatory elements comprise an epigenetic mechanism that enables the activation of neurogenic gene expression in adult NSCs throughout life.
Activation of Neuronal Gene Expression by the JMJD3 Demethylase Is Required for Postnatal and Adult Brain Neurogenesis / D.H. Park, S.J. Hong, R.D. Salinas, S.J. Liu, S.W. Sun, J. Sgualdino, G. Testa, M. Matzuk, N. Iwamori, D.A. Lim. - In: CELL REPORTS. - ISSN 2211-1247. - 8:5(2014), pp. 1290-1299.
|Titolo:||Activation of Neuronal Gene Expression by the JMJD3 Demethylase Is Required for Postnatal and Adult Brain Neurogenesis|
|Parole Chiave:||Animals; Brain; Cells, Cultured; Enhancer Elements, Genetic; Gene Expression Regulation, Developmental; HEK293 Cells; Homeodomain Proteins; Humans; Jumonji Domain-Containing Histone Demethylases; Mice; Neural Stem Cells; Promoter Regions, Genetic; Transcription Factors; Neurogenesis; Biochemistry, Genetics and Molecular Biology (all); Medicine (all)|
|Settore Scientifico Disciplinare:||Settore BIO/11 - Biologia Molecolare|
Settore BIO/13 - Biologia Applicata
Settore BIO/18 - Genetica
Settore BIO/10 - Biochimica
|Data di pubblicazione:||2014|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1016/j.celrep.2014.07.060|
|Appare nelle tipologie:||01 - Articolo su periodico|