On-chip single cell funneling operated by microfabricated thermo-responsive hydrogel layers

Santaniello, T.; Yan, Y.; Tocchio, A.; Martello, F.; Gassa, F.; Webb, P.; Zhao, W.; Tamplenizza, M.; Schulte, C.; Liu, Y.; Hutt, D.; Milani, P.; Conway, P.; Lenardi, C.

doi:10.1088/0960-1317/25/7/075004

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We present a multilayer microfluidic system having a KrF excimer laser micro-patterned thermo-responsive poly-(N-isopropyl)-acrylamide (PNIPAAm) based hydrogel layer integrated as a freestanding component that operates as a temperature-triggered cell isolation actuator for single cell assays applications. When the system is assembled, the size of the laser machined micro-through-hole (entrance diameter is 150 μm, while exit hole diameter varies from 10 to 80 μm) can be reversibly modulated as a consequence of the polymer volumetric phase transition induced by heating the device above the critical temperature of 32 °C; as a result of the polymer water loss, the shrinkage of the layer caused the hole to homogeneously shrink, thus reducing its original size to about 40% in the polymer collapsed state. This actuation mechanism was exploited to trap a cellular sample in the shrunken exit hole on the top of the hydrogel layer by applying a negative pressure across the film when the system is brought to 37 °C. Subsequently, the funneling of the trapped cell took place through the orifice when the polymer's natural relaxation at room temperature toward its initial state occurred; the functionality of the device was proved using optical microscopy to monitor MG63 cells as a model cell line during the funneling through the size-modulating structure.

On-chip single cell funneling operated by microfabricated thermo-responsive hydrogel layers / T. Santaniello, Y. Yan, A. Tocchio, F. Martello, F. Gassa, P. Webb, W. Zhao, M. Tamplenizza, C. Schulte, Y. Liu, D. Hutt, P. Milani, P. Conway, C. Lenardi. - In: JOURNAL OF MICROMECHANICS AND MICROENGINEERING. - ISSN 0960-1317. - 25:7(2015), pp. 075004.1-075004.12.

Titolo:	On-chip single cell funneling operated by microfabricated thermo-responsive hydrogel layers
Autori:	SANTANIELLO, TOMMASO (Primo) YAN, YUNSONG (Secondo) TOCCHIO, ALESSANDRO MARTELLO, FEDERICO F. Gassa P. Webb W. Zhao TAMPLENIZZA, MARGHERITA SCHULTE, CARSTEN Y. Liu D. Hutt MILANI, PAOLO P. Conway LENARDI, CRISTINA (Ultimo) mostra contributor esterni nascondi contributor esterni
Parole Chiave:	cells on a chip; micromachining; smart materials; Mechanical Engineering; Electrical and Electronic Engineering; Mechanics of Materials; Electronic, Optical and Magnetic Materials
Settore Scientifico Disciplinare:	Settore FIS/01 - Fisica Sperimentale Settore FIS/03 - Fisica della Materia
Data di pubblicazione:	2015
Rivista:	JOURNAL OF MICROMECHANICS AND MICROENGINEERING
Tipologia:	Article (author)
Digital Object Identifier (DOI):	http://dx.doi.org/10.1088/0960-1317/25/7/075004
Appare nelle tipologie:	01 - Articolo su periodico

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http://hdl.handle.net/2434/352800

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