Musculoskeletal diseases strongly influence life and performance of human beings and athletes, since tissue repair is often a slow and partial process. In athletic horses, musculoskeletal injuries of joints, ligaments, tendons, bone and cartilage, are frequent and they cause joint degeneration and osteoarthritis or osteochondrosis; in addition, right now their treatment often does not provide a full functional recovery. Cell-based therapies have shown encouraging results in tissue regeneration; here we show a pilot study, with autologous and allogeneic mesenchymal stem cells, isolated from equine adipose tissue (eqASCs), to treat equine musculoskeletal tissue disorders. eqASCs were isolated from fat tissue of 8 adult injured horses, with an easy and minimally invasive procedure. eqASCs were expanded and characterized in vitro. They proliferate fast (mean DT= 62,3±23,5) allowing a rapid production of a large number of cells (minimum 2x107 cells in 20 days); eqASCs are clonogenic (12,8±5,1%) and able to multi-differentiate (osteo-induced cells showed an increase in alkaline phosphatase activity of 493% compared to control cells). 10 horses (12,8±2,4 y/o) with injured tendon, ligament and articular cartilage were intra-lesion treated with 5x106 eqASCs. Interestingly, soon lameness reduction was observed; after 4 months, 9/10 horses had a good clinical outcome , and 8/10 horses started working again. At 8-21 months, one of the 8 active horses showed a further improvement, while one relapsed. These preliminary results confirm the safety of the in vitro expanded eqASCs (cryopreserved or not, and autologous or allogenic), and highlight their potential therapeutic use, related to their regenerative potential and, most likely, to their anti-inflammatory action on musculoskeletal tissues. In conclusion, also equine adipose tissue contains multipotent cells useful for veterinary cell therapy. However, further experiments with standardized protocols and more clinical cases are required to better elucidate their action and to create a network between vet and bio world.
A pilot study: effect of autologous and allogeneic equine adipose-derived stem cells in musculoskeletal diseases / L.M.J. Ferreira, S. Niada, F. Monici, A. Garbieri, C. Giannasi, A.T. Brini. ((Intervento presentato al convegno FIRST tenutosi a Milano nel 2014.
A pilot study: effect of autologous and allogeneic equine adipose-derived stem cells in musculoskeletal diseases
L.M.J. FerreiraPrimo
;S. Niada;C. Giannasi;A.T. Brini
2014
Abstract
Musculoskeletal diseases strongly influence life and performance of human beings and athletes, since tissue repair is often a slow and partial process. In athletic horses, musculoskeletal injuries of joints, ligaments, tendons, bone and cartilage, are frequent and they cause joint degeneration and osteoarthritis or osteochondrosis; in addition, right now their treatment often does not provide a full functional recovery. Cell-based therapies have shown encouraging results in tissue regeneration; here we show a pilot study, with autologous and allogeneic mesenchymal stem cells, isolated from equine adipose tissue (eqASCs), to treat equine musculoskeletal tissue disorders. eqASCs were isolated from fat tissue of 8 adult injured horses, with an easy and minimally invasive procedure. eqASCs were expanded and characterized in vitro. They proliferate fast (mean DT= 62,3±23,5) allowing a rapid production of a large number of cells (minimum 2x107 cells in 20 days); eqASCs are clonogenic (12,8±5,1%) and able to multi-differentiate (osteo-induced cells showed an increase in alkaline phosphatase activity of 493% compared to control cells). 10 horses (12,8±2,4 y/o) with injured tendon, ligament and articular cartilage were intra-lesion treated with 5x106 eqASCs. Interestingly, soon lameness reduction was observed; after 4 months, 9/10 horses had a good clinical outcome , and 8/10 horses started working again. At 8-21 months, one of the 8 active horses showed a further improvement, while one relapsed. These preliminary results confirm the safety of the in vitro expanded eqASCs (cryopreserved or not, and autologous or allogenic), and highlight their potential therapeutic use, related to their regenerative potential and, most likely, to their anti-inflammatory action on musculoskeletal tissues. In conclusion, also equine adipose tissue contains multipotent cells useful for veterinary cell therapy. However, further experiments with standardized protocols and more clinical cases are required to better elucidate their action and to create a network between vet and bio world.
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