Introduction Adipose-derived Stem Cells (ASCs) are multipotent, able of self-renewal, low immunogenic and with immunosuppressive properties. Additionally, ASCs have shown the capacity of limiting neuronal damage through an anti-apoptotic effect and releasing neurotrophic molecules. These features make ASCs an attractive tool for the treatment of pathology involving neuronal-tissue damage and inflammation, such as neuropathic pain. Therefore we studied the effect of human ASCs in a mouse sciatic nerve chronic constriction injury (CCI) model. Methods hASC were isolated from subcutaneous adipose tissue of 5 healthy women (age 37±12); all the populations were phenotipically characterized and their clonogenic and differentiative potential evaluated to verify their stemness. 10^6 hASC were e.v. injected into C57BL/6 mice 7 days after CCI of the sciatic nerve, and, at day 1,3,7,14,21 and 28 post injection, we assessed their effect on mechanical allodynia and thermal hyperalgesia and correlated it with pro- and anti-inflammatory cytokines levels. We have also injected 10^6 or 5X10^5 cells twice, and their effects were also monitored. Results hASCs have been characterized for their stemness by standard assays while growing them for the animals’ treatment. All the injected animals survive to the treatment and no abnormal behaviors were observed. Already 24 hours after injection, hASCs were able to completely reverse hyperalgesia and to reduce allodynia; the intensity of this effect was correlated to the number of injected cells, beginning to fade 21 days after cell treatment, and it could be restored by a new treatment. In addition, the anti-hyperalgesic effect was hASCs-specific, since treatment with murine fibroblast was un-effective. At the level of the lesion site, we have also noticed that cytokines balance both for pro- (IL-1β) and anti-inflammatory ones (IL-10)was restored by hASCs. Conclusions Here we have shown that expanded hASCs reduce neuropathic pain symptoms in a CCI mouse model. The mechanism needs to be elucidated, however we have shown that the cytokine balance might be re-established in the lesioned tissue, confirming an anti-inflammatory role of these cells which could be exploited for the treatment of other inflammatory pathologies.
|Titolo:||Effect of human adipose-derived stem cells treatment in a mouse model of neuropathic pain|
|Data di pubblicazione:||2013|
|Settore Scientifico Disciplinare:||Settore BIO/13 - Biologia Applicata|
|Citazione:||Effect of human adipose-derived stem cells treatment in a mouse model of neuropathic pain / S. Niada, A. Rossi, E. Arrigoni, S. Franchi, A.E. Panerai, P. Sacerdote, A.T. Brini. ((Intervento presentato al convegno IFATS tenutosi a New York nel 2013.|
|Appare nelle tipologie:||14 - Intervento a convegno non pubblicato|