ABSTRACT Valentina Crippa Cell Blocks from canine and feline effusions Objective: To evaluate the sensitivity and specificity of a panel of markers in distinguishing mesothelial cells from metastatic adenocarcinoma cells in Cell blocks from canine and feline effusion. Methods: This study included 28 effusion specimens from dogs and cats with a cytological diagnosis of reactive effusion or malignancy of non-hematopoietic origin. Cell Blocks were stained with a standard panel of Vimentin, panCK (MNF116), CK 5/6 and HBME-1 as mesothelial cell markers; Desmin as marker of benign mesothelial cells; Claudin 4 as epithelial marker and CK7/CK20 as a marker of metastasis. Malignancy was confirmed by histologic evaluation; non-malignant conditions were confirmed by histopathology or follow up. Sensitivities, specificities, predictive values and accuracy were calculated. Results: CK5/6 demonstrated a high specificity (100%) for mesothelium. For the detection of canine and feline mesothelial cells the coexpression of panCK and VIM displayed the best sensibility (94,1%) while HBME-1 was the antibody that presented highest accuracy. Claudin 4 demonstrated a very low sensibility versus canine and feline epithelial cells. Conclusion: The most specific marker, with for the identification of mesothelial cells in canine and effusion, is the Vim/CK coexpression, being CK5/6 the most specific and HBME-1 the marker with the highest overall accuracy. Desmin is a useful marker in discriminating between benign and malignant mesothelial cells. The coordinate expression of CK7/CK20+ has not proved to be useful on the identification of metastatic cells on effusion. The study of Claudin 4 necessitate to be deepened in veterinary medicine. In conclusion, the combination of both cytology and immunohistochemistry studies can greatly enhance the diagnostic accuracy, sensitivity and specificity in malignant effusions.
CELL BLOCKS OF CANINE AND FELINE EFFUSION / V. Crippa ; tutor: M. Caniatti ; coordinatore: G. Sironi. - Milano : Università degli studi di Milano. DIPARTIMENTO DI SCIENZE VETERINARIE E SANITA' PUBBLICA, 2016 Jan 19. ((27. ciclo, Anno Accademico 2015.
|Titolo:||CELL BLOCKS OF CANINE AND FELINE EFFUSION|
|Supervisori e coordinatori interni:||SIRONI, GIUSEPPE|
|Data di pubblicazione:||19-gen-2016|
|Settore Scientifico Disciplinare:||Settore VET/03 - Patologia Generale e Anatomia Patologica Veterinaria|
|Citazione:||CELL BLOCKS OF CANINE AND FELINE EFFUSION / V. Crippa ; tutor: M. Caniatti ; coordinatore: G. Sironi. - Milano : Università degli studi di Milano. DIPARTIMENTO DI SCIENZE VETERINARIE E SANITA' PUBBLICA, 2016 Jan 19. ((27. ciclo, Anno Accademico 2015.|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.13130/crippa-valentina_phd2016-01-19|
|Appare nelle tipologie:||Tesi di dottorato|