Communication between cardiomyocytes depends upon gap junctions (GJ). Previous studies have demonstrated that electrical stimulation induces GJ remodeling and modifies histone acetylase (HAT) and deacetylase (HDAC) activities, although these two results have not been linked. The aim of this work was to establish whether electrical stimulation modulates GJ-mediated cardiac cell-cell communication by acetylation-dependent mechanisms. Field stimulation of HL-1 cardiomyocytes at 0.5. Hz for 24. h significantly reduced connexin43 (Cx43) expression and cell-cell communication. HDAC activity was down-regulated whereas HAT activity was not modified resulting in increased acetylation of Cx43. Consistent with a post-translational mechanism, we did not observe a reduction in Cx43 mRNA in electrically stimulated cells, while the proteasomal inhibitor MG132 maintained Cx43 expression. Further, the treatment of paced cells with the HAT inhibitor Anacardic Acid maintained both the levels of Cx43 and cell-cell communication. Finally, we observed increased acetylation of Cx43 in the left ventricles of dogs subjected to chronic tachypacing as a model of abnormal ventricular activation.In conclusion, our findings suggest that altered electrical activity can regulate cardiomyocyte communication by influencing the acetylation status of Cx43.

Acetylation mediates Cx43 reduction caused by electrical stimulation / V. Meraviglia, V. Azzimato, C. Colussi, M.C. Florio, A. Binda, A. Panariti, K. Qanud, S. Suffredini, L. Gennaccaro, M. Miragoli, A. Barbuti, P.D. Lampe, C. Gaetano, P.P. Pramstaller, M.C. Capogrossi, F.A. Recchia, G. Pompilio, I. Rivolta, A. Rossini. - In: JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY. - ISSN 0022-2828. - 87(2015 Oct), pp. 54-64.

Acetylation mediates Cx43 reduction caused by electrical stimulation

A. Barbuti;G. Pompilio;
2015

Abstract

Communication between cardiomyocytes depends upon gap junctions (GJ). Previous studies have demonstrated that electrical stimulation induces GJ remodeling and modifies histone acetylase (HAT) and deacetylase (HDAC) activities, although these two results have not been linked. The aim of this work was to establish whether electrical stimulation modulates GJ-mediated cardiac cell-cell communication by acetylation-dependent mechanisms. Field stimulation of HL-1 cardiomyocytes at 0.5. Hz for 24. h significantly reduced connexin43 (Cx43) expression and cell-cell communication. HDAC activity was down-regulated whereas HAT activity was not modified resulting in increased acetylation of Cx43. Consistent with a post-translational mechanism, we did not observe a reduction in Cx43 mRNA in electrically stimulated cells, while the proteasomal inhibitor MG132 maintained Cx43 expression. Further, the treatment of paced cells with the HAT inhibitor Anacardic Acid maintained both the levels of Cx43 and cell-cell communication. Finally, we observed increased acetylation of Cx43 in the left ventricles of dogs subjected to chronic tachypacing as a model of abnormal ventricular activation.In conclusion, our findings suggest that altered electrical activity can regulate cardiomyocyte communication by influencing the acetylation status of Cx43.
English
Acetylation; Connexin43; Electrical field stimulation; Gap junctions; Molecular Biology; Cardiology and Cardiovascular Medicine
Settore BIO/11 - Biologia Molecolare
Articolo
Esperti anonimi
Pubblicazione scientifica
ott-2015
Academic Press
87
54
64
11
Pubblicato
Periodico con rilevanza internazionale
scopus
pubmed
crossref
Aderisco
info:eu-repo/semantics/article
Acetylation mediates Cx43 reduction caused by electrical stimulation / V. Meraviglia, V. Azzimato, C. Colussi, M.C. Florio, A. Binda, A. Panariti, K. Qanud, S. Suffredini, L. Gennaccaro, M. Miragoli, A. Barbuti, P.D. Lampe, C. Gaetano, P.P. Pramstaller, M.C. Capogrossi, F.A. Recchia, G. Pompilio, I. Rivolta, A. Rossini. - In: JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY. - ISSN 0022-2828. - 87(2015 Oct), pp. 54-64.
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Prodotti della ricerca::01 - Articolo su periodico
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262
Article (author)
si
V. Meraviglia, V. Azzimato, C. Colussi, M.C. Florio, A. Binda, A. Panariti, K. Qanud, S. Suffredini, L. Gennaccaro, M. Miragoli, A. Barbuti, P.D. Lamp...espandi
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/346324
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