BACKGROUND: To evaluate the safety of sperm washing and assisted reproduction technique (ART) outcome offered to serodiscordant couples with a human immunodeficiency virus-1 (HIV-1)-positive male. METHODS: Sperm washing was performed and checked by RT-PCR on each semen sample before its fresh usage. Intrauterine insemination (IUI) or IFV/ICSI was offered according to fertility profile of each couple. Non-infected women underwent HIV testing 2 weeks before each procedure and for up to 6 months after. RESULTS: Seven hundred and forty-one couples entered the study of a possible 2011 serodiscordant couples counselled over 4 years. Superovulation and IUI were performed in 581 couples, where the pregnancy rate per cycle and pregnancy rate per couple were 19 and 78%, respectively, with multiple pregnancy rate being 4%. One hundred and sixty couples were treated by IVF/ICSI, where pregnancy rate per cycle and per couple were 22 and 41%, respectively, with multiple pregnancy rate being 10%. All female partners were still HIV-1 negative at follow-up. CONCLUSION: Sperm washing within a programme of reproductive counselling was proved to be safe in this large series of serodiscordant couples. The overall pregnancy rate (70.3%), independent of the procedure used (IUI or IVF/ICSI), justifies the effort of the medical team in setting up and implementing dedicated centres and of the individual patient in seeking a safe pregnancy.
Safety of sperm washing and ART outcome in 741 HIV-1-serodiscordant couples / V.M. Savasi, E.M. Ferrazzi, C. Lanzani, M. Oneta, B. Parrilla, T. Persico. - In: HUMAN REPRODUCTION. - ISSN 0268-1161. - 22:3(2007), pp. 772-777.
|Titolo:||Safety of sperm washing and ART outcome in 741 HIV-1-serodiscordant couples|
SAVASI, VALERIA MARIA (Primo)
FERRAZZI, ENRICO MARIO (Secondo)
PERSICO, TIZIANA (Ultimo)
|Parole Chiave:||ART; HIV; RT-PCR; Serodiscordant couples; Treatment outcome|
|Settore Scientifico Disciplinare:||Settore MED/40 - Ginecologia e Ostetricia|
|Data di pubblicazione:||2007|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1093/humrep/del422|
|Appare nelle tipologie:||01 - Articolo su periodico|