Upon activation, lymphocytes release vesicles containing microRNAs (miRNAs). However, little is known as to whether this release results in modulation of circulating miRNAs (the miRNome) in the serum. The present work aims to identify lymphocyte subset-specific signatures of miRNAs within the serum circulating miRNome. We therefore assessed serum miRNA expression profiles in wild type mice; in mice lacking either CD4(+) T cells, CD8(+) T cells, invariant Natural Killer T (iNKT) cells or B cells; and, as a control, in mice in which Dicer has been ablated in T lymphocytes. We found that specific serum miRNAs are differentially modulated when different lymphocyte subsets are lacking. In particular, the serum level of miR-181b-5p, previously demonstrated to be fundamental for the development of iNKT cells, is specifically reduced in mice in which iNKT cells are absent. Interestingly, our results indicate a direct link between the biological role of a single miRNA in lymphocyte development and its serum level, and prove that even a population composed of relatively few cells in vivo, such as iNKT lymphocytes, has a measurable effect on the serum circulating miRNome.

The circulating microRNome demonstrates distinct lymphocyte subset-dependent signatures / P. de Candia, A. Torri, M. Fedeli, V. Viganò, D. Carpi, T. Gorletta, G. Casorati, M. Pagani, P. Dellabona, S. Abrignani. - In: EUROPEAN JOURNAL OF IMMUNOLOGY. - ISSN 0014-2980. - 46:3(2016 Mar), pp. 725-731. [10.1002/eji.201545787]

The circulating microRNome demonstrates distinct lymphocyte subset-dependent signatures

M. Pagani;S. Abrignani
Ultimo
2016

Abstract

Upon activation, lymphocytes release vesicles containing microRNAs (miRNAs). However, little is known as to whether this release results in modulation of circulating miRNAs (the miRNome) in the serum. The present work aims to identify lymphocyte subset-specific signatures of miRNAs within the serum circulating miRNome. We therefore assessed serum miRNA expression profiles in wild type mice; in mice lacking either CD4(+) T cells, CD8(+) T cells, invariant Natural Killer T (iNKT) cells or B cells; and, as a control, in mice in which Dicer has been ablated in T lymphocytes. We found that specific serum miRNAs are differentially modulated when different lymphocyte subsets are lacking. In particular, the serum level of miR-181b-5p, previously demonstrated to be fundamental for the development of iNKT cells, is specifically reduced in mice in which iNKT cells are absent. Interestingly, our results indicate a direct link between the biological role of a single miRNA in lymphocyte development and its serum level, and prove that even a population composed of relatively few cells in vivo, such as iNKT lymphocytes, has a measurable effect on the serum circulating miRNome.
circulating microRNAs; lymphocyte subsets; iNKT lymphocytes
Settore BIO/11 - Biologia Molecolare
29-dic-2015
Article (author)
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/344828
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