Arginine is a semi-essential amino acid that plays an important role in the regulation of metabolic processes associated with several pathological/ physiological conditions. In the vasculature, it mainly exerts its biological functions as a substrate of two alternative pathways: the conversion to nitric oxide (NO) by nitric oxide synthase (NOS) and the breakdown to urea and ornithine by arginase. To determine arginine metabolism, in the current study we propose an original radiochemical technique that allows the simultaneous monitoring of NOS and arginase activation within intact cells. Taking advantage of this method, we show here the consequences of different experimental conditions known to modulate endothelial homeostasis on arginine metabolism.
Radiochemical high-performance liquid chromatography detection of arginine metabolism in human endothelial cells / A. Barilli, R. Visigalli, B.M. Rotoli, O. Bussolati, G.C. Gazzola, A. Parolari, V. Dall'Asta. - In: ANALYTICAL BIOCHEMISTRY. - ISSN 0003-2697. - 424:2(2012), pp. 156-161. [10.1016/j.ab.2012.02.023]
Radiochemical high-performance liquid chromatography detection of arginine metabolism in human endothelial cells
A. ParolariPenultimo
;
2012
Abstract
Arginine is a semi-essential amino acid that plays an important role in the regulation of metabolic processes associated with several pathological/ physiological conditions. In the vasculature, it mainly exerts its biological functions as a substrate of two alternative pathways: the conversion to nitric oxide (NO) by nitric oxide synthase (NOS) and the breakdown to urea and ornithine by arginase. To determine arginine metabolism, in the current study we propose an original radiochemical technique that allows the simultaneous monitoring of NOS and arginase activation within intact cells. Taking advantage of this method, we show here the consequences of different experimental conditions known to modulate endothelial homeostasis on arginine metabolism.File | Dimensione | Formato | |
---|---|---|---|
2012 Anal Biochem.pdf
accesso riservato
Tipologia:
Publisher's version/PDF
Dimensione
673.54 kB
Formato
Adobe PDF
|
673.54 kB | Adobe PDF | Visualizza/Apri Richiedi una copia |
Pubblicazioni consigliate
I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.