A human glioblastoma multiforme cell line (U87) and its derived-spheroids were irradiated either using a conventional irradiation (CIR) or a CK-like irradiation (IIR) in which the 8 Gy was delivered intermittently over a period of 40 min. The ability of glioma cells to migrate into a matrigel matrix was evaluated on days 1-8 from irradiation. Irradiation with CK-driven IIR significantly increased the invasion potential of U87 cells in a matrigel-based assay. In contrast to CIR, IIR was associated with increased levels of TGF beta at four days (real-time PCR), beta 1-integrin at 4-5 d (real-time PCR and protein gel blot) and no elevation in phosphorylated AKT at days 4 and 5 (protein gel blot). Our data suggests that glioma cell invasion as well as elevations of TGF beta and beta 1-integrin are associated with IIR and not CIR.
Increased migration of a human glioma cell line after in vitro CyberKnife irradiation / A. Canazza, C. Calatozzolo, L. Fumagalli, A. Bergantin, F. Ghielmetti, L. Fariselli, D. Croci, A. Salmaggi, E. Ciusani. - In: CANCER BIOLOGY & THERAPY. - ISSN 1538-4047. - 12:7(2011 Oct 01), pp. 629-633. [10.4161/cbt.12.7.16862]
Increased migration of a human glioma cell line after in vitro CyberKnife irradiation
A. Canazza;A. Bergantin;F. Ghielmetti;
2011
Abstract
A human glioblastoma multiforme cell line (U87) and its derived-spheroids were irradiated either using a conventional irradiation (CIR) or a CK-like irradiation (IIR) in which the 8 Gy was delivered intermittently over a period of 40 min. The ability of glioma cells to migrate into a matrigel matrix was evaluated on days 1-8 from irradiation. Irradiation with CK-driven IIR significantly increased the invasion potential of U87 cells in a matrigel-based assay. In contrast to CIR, IIR was associated with increased levels of TGF beta at four days (real-time PCR), beta 1-integrin at 4-5 d (real-time PCR and protein gel blot) and no elevation in phosphorylated AKT at days 4 and 5 (protein gel blot). Our data suggests that glioma cell invasion as well as elevations of TGF beta and beta 1-integrin are associated with IIR and not CIR.File | Dimensione | Formato | |
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