A human glioblastoma multiforme cell line (U87) and its derived-spheroids were irradiated either using a conventional irradiation (CIR) or a CK-like irradiation (IIR) in which the 8 Gy was delivered intermittently over a period of 40 min. The ability of glioma cells to migrate into a matrigel matrix was evaluated on days 1-8 from irradiation. Irradiation with CK-driven IIR significantly increased the invasion potential of U87 cells in a matrigel-based assay. In contrast to CIR, IIR was associated with increased levels of TGF beta at four days (real-time PCR), beta 1-integrin at 4-5 d (real-time PCR and protein gel blot) and no elevation in phosphorylated AKT at days 4 and 5 (protein gel blot). Our data suggests that glioma cell invasion as well as elevations of TGF beta and beta 1-integrin are associated with IIR and not CIR.
|Titolo:||Increased migration of a human glioma cell line after in vitro CyberKnife irradiation|
|Parole Chiave:||glioma; radiation; clonogenic survival; invasion; CyberKnife|
|Settore Scientifico Disciplinare:||Settore BIO/13 - Biologia Applicata|
|Data di pubblicazione:||1-ott-2011|
|Digital Object Identifier (DOI):||10.4161/cbt.12.7.16862|
|Appare nelle tipologie:||01 - Articolo su periodico|