A human glioblastoma multiforme cell line (U87) and its derived-spheroids were irradiated either using a conventional irradiation (CIR) or a CK-like irradiation (IIR) in which the 8 Gy was delivered intermittently over a period of 40 min. The ability of glioma cells to migrate into a matrigel matrix was evaluated on days 1-8 from irradiation. Irradiation with CK-driven IIR significantly increased the invasion potential of U87 cells in a matrigel-based assay. In contrast to CIR, IIR was associated with increased levels of TGF beta at four days (real-time PCR), beta 1-integrin at 4-5 d (real-time PCR and protein gel blot) and no elevation in phosphorylated AKT at days 4 and 5 (protein gel blot). Our data suggests that glioma cell invasion as well as elevations of TGF beta and beta 1-integrin are associated with IIR and not CIR.
Increased migration of a human glioma cell line after in vitro CyberKnife irradiation / A. Canazza, C. Calatozzolo, L. Fumagalli, A. Bergantin, F. Ghielmetti, L. Fariselli, D. Croci, A. Salmaggi, E. Ciusani. - In: CANCER BIOLOGY & THERAPY. - ISSN 1538-4047. - 12:7(2011 Oct 01), pp. 629-633.
Titolo: | Increased migration of a human glioma cell line after in vitro CyberKnife irradiation |
Autori: | |
Parole Chiave: | glioma; radiation; clonogenic survival; invasion; CyberKnife |
Settore Scientifico Disciplinare: | Settore BIO/13 - Biologia Applicata |
Data di pubblicazione: | 1-ott-2011 |
Rivista: | |
Tipologia: | Article (author) |
Digital Object Identifier (DOI): | http://dx.doi.org/10.4161/cbt.12.7.16862 |
Appare nelle tipologie: | 01 - Articolo su periodico |
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