Long noncoding RNAs are emerging as important regulators of cellular functions, but little is known of their role in the human immune system. Here we investigated long intergenic noncoding RNAs (lincRNAs) in 13 subsets of T lymphocytes and B lymphocytes by next-generation sequencing–based RNA sequencing (RNA-seq analysis) and de novo transcriptome reconstruction. We identified over 500 previously unknown lincRNAs and described lincRNA signatures. Expression of linc-MAF-4, a chromatin-associated lincRNA specific to the TH1 subset of helper T cells, was inversely correlated with expression of MAF, a TH2-associated transcription factor. Downregulation of linc-MAF-4 skewed T cell differentiation toward the TH2 phenotype. We identified a long-distance interaction between the genomic regions of the gene encoding linc-MAF-4 and MAF, where linc-MAF-4 associated with the chromatin modifiers LSD1 and EZH2; this suggested that linc-MAF-4 regulated MAF transcription through the recruitment of chromatin modifiers. Our results demonstrate a key role for lincRNA in T lymphocyte differentiation.

The long intergenic noncoding RNA landscape of human lymphocytes highlights the regulation of T cell differentiation by linc-MAF-4 / V. Ranzani, G. Rossetti, I. Panzeri, A. Arrigoni, R. Bonnal, S. Curti, P. Gruarin, E. Provasi, E. Sugliano, M. Marconi, R. De Francesco, J. Geginat, B. Bodega, S. Abrignani, M. Pagani. - In: NATURE IMMUNOLOGY. - ISSN 1529-2908. - 16(2015 Mar 16), pp. 318-325.

The long intergenic noncoding RNA landscape of human lymphocytes highlights the regulation of T cell differentiation by linc-MAF-4

R. De Francesco;J. Geginat;B. Bodega;S. Abrignani
;
M. Pagani
2015

Abstract

Long noncoding RNAs are emerging as important regulators of cellular functions, but little is known of their role in the human immune system. Here we investigated long intergenic noncoding RNAs (lincRNAs) in 13 subsets of T lymphocytes and B lymphocytes by next-generation sequencing–based RNA sequencing (RNA-seq analysis) and de novo transcriptome reconstruction. We identified over 500 previously unknown lincRNAs and described lincRNA signatures. Expression of linc-MAF-4, a chromatin-associated lincRNA specific to the TH1 subset of helper T cells, was inversely correlated with expression of MAF, a TH2-associated transcription factor. Downregulation of linc-MAF-4 skewed T cell differentiation toward the TH2 phenotype. We identified a long-distance interaction between the genomic regions of the gene encoding linc-MAF-4 and MAF, where linc-MAF-4 associated with the chromatin modifiers LSD1 and EZH2; this suggested that linc-MAF-4 regulated MAF transcription through the recruitment of chromatin modifiers. Our results demonstrate a key role for lincRNA in T lymphocyte differentiation.
No
English
Settore BIO/11 - Biologia Molecolare
Articolo
Esperti anonimi
Pubblicazione scientifica
   Lymphocyte microRNAs in health and disease: Understanding lymphocyte functions through the identification of microRNA target genes and exploiting serum microRNA signatures to monitor immune responses
   I-MIRNOME
   EUROPEAN COMMISSION
   FP7
   269022

   Long non-coding RNAs of tumor infiltrating lymphocytes as novel anti-cancer therapeutic targets
   FIGHT-CANCER
   EUROPEAN COMMISSION
   FP7
   617978
16-mar-2015
26-gen-2015
Nature
16
318
325
8
Pubblicato
Periodico con rilevanza internazionale
Aderisco
info:eu-repo/semantics/article
The long intergenic noncoding RNA landscape of human lymphocytes highlights the regulation of T cell differentiation by linc-MAF-4 / V. Ranzani, G. Rossetti, I. Panzeri, A. Arrigoni, R. Bonnal, S. Curti, P. Gruarin, E. Provasi, E. Sugliano, M. Marconi, R. De Francesco, J. Geginat, B. Bodega, S. Abrignani, M. Pagani. - In: NATURE IMMUNOLOGY. - ISSN 1529-2908. - 16(2015 Mar 16), pp. 318-325.
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Prodotti della ricerca::01 - Articolo su periodico
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Article (author)
si
V. Ranzani, G. Rossetti, I. Panzeri, A. Arrigoni, R. Bonnal, S. Curti, P. Gruarin, E. Provasi, E. Sugliano, M. Marconi, R. De Francesco, J. Geginat, B...espandi
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/341464
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