Several procedures to isolate DNA from difficult sources have previously been described, but they are often expensive, time consuming, and have limited applications. In this paper we describe a simple and versatile protocol to isolate nucleic acids from different plant tissues, using a silica-based extraction method. This extraction process efficiently purifies DNA from several plant cells. The obtained DNA has successfully been applied in polymerase chain reaction (PCR) assays for OGM purposes. The protocol is compatible with at least two automated liquid handling systems making it suitable for large-scale screening applications.
A simple extraction method useful to purify DNA from difficult biologic sources / Claudio Oliveri, Melissa Frequin, Giulia Malferrari, Giuliana Saltini, Maurizio Gramegna, Roberta Tagliabue, Pasquale De Blasio, Ida Biunno, Laura Biagiotti. - In: CELL PRESERVATION TECHNOLOGY. - ISSN 1538-344X. - 4:1(2006 Mar), pp. 51-54. [10.1089/cpt.2006.4.51]
A simple extraction method useful to purify DNA from difficult biologic sources
C.L. Oliveri;M.C. Frequin;G.L. Malferrari;G.L.M. Saltini;L. Biagiotti
2006
Abstract
Several procedures to isolate DNA from difficult sources have previously been described, but they are often expensive, time consuming, and have limited applications. In this paper we describe a simple and versatile protocol to isolate nucleic acids from different plant tissues, using a silica-based extraction method. This extraction process efficiently purifies DNA from several plant cells. The obtained DNA has successfully been applied in polymerase chain reaction (PCR) assays for OGM purposes. The protocol is compatible with at least two automated liquid handling systems making it suitable for large-scale screening applications.
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