My PhD project addressed the paternal and maternal contribution associated with correct early embryonic development, focusing on microRNAs (miRNAs, short non-coding RNAs regulating gene expression at post-transcriptional level) involved in controlling reproductive function in cattle. To this end, next generation sequencing (NGS) methods and bioinformatic analysis pipelines were set up and validated using low quantities of starting materials, e.g. blastocysts and oocytes. The role of miRNAs carried by spermatozoa during early embryogenesis was investigated by assessing in vitro produced blastocysts from semen of bulls with high and low blastocyst rates. Moreover, follicular fluid and oocyte miRNAs were analyzed in order to unravel molecular mechanisms involved in poor fertility cows. In particular, a model based on low and high antral follicle count ovaries was used to study the influence of ovarian function on oocyte developmental competence. The results indicated that: 1) sperm miRNAs may impact embryo developmental competence affecting the expression of gene networks involved in several cellular processes including cell adhesion, communication and metabolism of the blastocyst; 2) the different oocyte quality is associated with a different miRNA blueprint in both follicular fluids and oocytes through the mis-regulation of biological processes critical for oocyte competence. In conclusion, these studies contributed to improve our knowledge on the function of miRNAs in the sperm, oocytes and pre-implantation embryos. Several miRNAs were identified as potential biomarkers of blastocyst and oocyte competences, which will be valuable to optimize Reproductive Biotechnologies.

MOLECULAR CHARACTERIZATION OF FACTORS WHICH CAN INFLUENCE THE REPRODUCTIVE SUCCESS OF CATTLE / R. Pasquariello ; tutor: F. Gandolfi, J.L. Williams ; coordinatore: F. Gandolfi. - : . DIPARTIMENTO DI SCIENZE AGRARIE E AMBIENTALI - PRODUZIONE, TERRITORIO, AGROENERGIA, 2015 Dec 15. ((28. ciclo, Anno Accademico 2015. [10.13130/r-pasquariello_phd2015-12-15].

MOLECULAR CHARACTERIZATION OF FACTORS WHICH CAN INFLUENCE THE REPRODUCTIVE SUCCESS OF CATTLE

R. Pasquariello
2015-12-15

Abstract

My PhD project addressed the paternal and maternal contribution associated with correct early embryonic development, focusing on microRNAs (miRNAs, short non-coding RNAs regulating gene expression at post-transcriptional level) involved in controlling reproductive function in cattle. To this end, next generation sequencing (NGS) methods and bioinformatic analysis pipelines were set up and validated using low quantities of starting materials, e.g. blastocysts and oocytes. The role of miRNAs carried by spermatozoa during early embryogenesis was investigated by assessing in vitro produced blastocysts from semen of bulls with high and low blastocyst rates. Moreover, follicular fluid and oocyte miRNAs were analyzed in order to unravel molecular mechanisms involved in poor fertility cows. In particular, a model based on low and high antral follicle count ovaries was used to study the influence of ovarian function on oocyte developmental competence. The results indicated that: 1) sperm miRNAs may impact embryo developmental competence affecting the expression of gene networks involved in several cellular processes including cell adhesion, communication and metabolism of the blastocyst; 2) the different oocyte quality is associated with a different miRNA blueprint in both follicular fluids and oocytes through the mis-regulation of biological processes critical for oocyte competence. In conclusion, these studies contributed to improve our knowledge on the function of miRNAs in the sperm, oocytes and pre-implantation embryos. Several miRNAs were identified as potential biomarkers of blastocyst and oocyte competences, which will be valuable to optimize Reproductive Biotechnologies.
GANDOLFI, FULVIO
GANDOLFI, FULVIO
Settore VET/01 - Anatomia degli Animali Domestici
MOLECULAR CHARACTERIZATION OF FACTORS WHICH CAN INFLUENCE THE REPRODUCTIVE SUCCESS OF CATTLE / R. Pasquariello ; tutor: F. Gandolfi, J.L. Williams ; coordinatore: F. Gandolfi. - : . DIPARTIMENTO DI SCIENZE AGRARIE E AMBIENTALI - PRODUZIONE, TERRITORIO, AGROENERGIA, 2015 Dec 15. ((28. ciclo, Anno Accademico 2015. [10.13130/r-pasquariello_phd2015-12-15].
Doctoral Thesis
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2434/335298
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