In the search of new drug delivery carriers for the brain, self-assembled nanoparticles (NP) were prepared from poly(N,N-dimethylacrylamide)-block-polystyrene polymer. NP displayed biocompatibility on cultured endothelial cells, macrophages and differentiated SH-SY5Y neuronal-like cells. The surface-functionalization of NP with a modified fragment of human Apolipoprotein E (mApoE) enhanced the uptake of NP by cultured human brain capillary endothelial cells, as assessed by confocal microscopy, and their permeability through a Transwell Blood Brain Barrier model made with the same cells, as assessed by fluorescence. Finally, mApoE-NP embedding doxorubicin displayed an enhanced release of drug at low pH, suggesting the potential use of these NP for the treatment of brain tumors.
Investigation of Functionalized Poly(N,N-dimethylacrylamide)-block-polystyrene Nanoparticles As Novel Drug Delivery System to Overcome the Blood-Brain Barrier In Vitro / M. Gregori, D. Bertani, E. Cazzaniga, A. Orlando, M. Mauri, A. Bianchi, F. Re, S. Sesana, S. Minniti, M. Francolini, A. Cagnotto, M. Salmona, L. Nardo, D. Salerno, F. Mantegazza, M. Masserini, R. Simonutti. - In: MACROMOLECULAR BIOSCIENCE. - ISSN 1616-5187. - 15:12(2015 Dec), pp. 1687-1697.
Titolo: | Investigation of Functionalized Poly(N,N-dimethylacrylamide)-block-polystyrene Nanoparticles As Novel Drug Delivery System to Overcome the Blood-Brain Barrier In Vitro |
Autori: | |
Parole Chiave: | blood-brain barrier; controlled drug release; copolymers; drug delivery; nanoparticles; biotechnology; bioengineering; biomaterials; polymers and plastics; materials chemistry2506 metals and alloys |
Settore Scientifico Disciplinare: | Settore BIO/14 - Farmacologia |
Data di pubblicazione: | dic-2015 |
Rivista: | |
Tipologia: | Article (author) |
Digital Object Identifier (DOI): | http://dx.doi.org/10.1002/mabi.201500172 |
Appare nelle tipologie: | 01 - Articolo su periodico |
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