Introduction: Risk factors for life-threatening cardiovascular events were evaluated in an experimental model of epilepsy, the Wistar Audiogenic Rat (WAR) strain. Methods: We used long-term ECG recordings in conscious, one year old, WAR and Wistar control counterparts to evaluate spontaneous arrhythmias and heart rate variability, a tool to assess autonomic cardiac control. Ventricular function was also evaluated using the pressure-volume conductance system in anesthetized rats. Results: Basal RR interval (RRi) was similar between WAR and Wistar rats (188±5 vs 199±6 ms). RRi variability strongly suggests that WAR present an autonomic imbalance with sympathetic overactivity, which is an isolated risk factor for cardiovascular events. Anesthetized WAR showed lower arterial pressure (92±3 vs 115±5 mmHg) and exhibited indices of systolic dysfunction, such as higher ventricle end-diastolic pressure (9.2±0.6 vs 5.6±1 mmHg) and volume (137±9 vs 68±9 μL) as well as lower rate of increase in ventricular pressure (5266±602 vs 7320±538 mmHg.s-1). Indices of diastolic cardiac function, such as lower rate of decrease in ventricular pressure (-5014±780 vs -7766±998 mmHg.s<sup>-1</sup>) and a higher slope of the linear relationship between end-diastolic pressure and volume (0.078±0.011 vs 0.036±0.011 mmHg.μL), were also found in WAR as compared to Wistar control rats. Moreover, Wistar rats had 3 to 6 ventricular ectopic beats, whereas WAR showed 15 to 30 ectopic beats out of the 20,000 beats analyzed in each rat. Conclusions: The autonomic imbalance observed previously at younger age is also present in aged WAR and, additionally, a cardiac dysfunction was also observed in the rats. These findings make this experimental model of epilepsy a valuable tool to study risk factors for cardiovascular events in epilepsy.
Evaluation of cardiovascular risk factors in the Wistar Audiogenic Rat (WAR) Strain / R. Fazan, C.A.A. Silva, J.A.C. Oliveira, H.C. Salgado, N. Montano, N. Garcia-Cairasco. - In: PLOS ONE. - ISSN 1932-6203. - 10:6(2015), pp. e0129574.1-e0129574.5.
|Titolo:||Evaluation of cardiovascular risk factors in the Wistar Audiogenic Rat (WAR) Strain|
MONTANO, NICOLA (Penultimo)
|Parole Chiave:||Agricultural and Biological Sciences (all); Biochemistry, Genetics and Molecular Biology (all); Medicine (all)|
|Settore Scientifico Disciplinare:||Settore MED/09 - Medicina Interna|
Settore MED/11 - Malattie dell'Apparato Cardiovascolare
|Data di pubblicazione:||2015|
|Data ahead of print / Data di stampa:||2015|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1371/journal.pone.0129574|
|Appare nelle tipologie:||01 - Articolo su periodico|