The organic alcohol 2-methyl-2,4-pentanediol, which is used as cosolvent in the aqueous buffer to induce crystallization of ascorbate oxidase, acts as a competitive inhibitor of ascorbic acid in the oxidase reaction. Glycerol and ethylene glycol show similar, but smaller inhibitory effects. The lower activity exhibited by crystalline ascorbate oxidase with respect to the native enzyme has been shown to depend on the ageing of the proteine solution during crystallization and not by specific effects due to the presence of 2-methyl-2,4-pentanediol.
Inhibition of Ascorbate Oxidase by 2-methyl-2,4-pentanediol and other polyols / L. Santagostini, M. Gullotti, F. Mutti. - In: CHEMISTRY: AN INDIAN JOURNAL. - ISSN 0972-8376. - 3:12(2006), pp. 411-416.
Inhibition of Ascorbate Oxidase by 2-methyl-2,4-pentanediol and other polyols
L. SantagostiniPrimo
;M. GullottiSecondo
;
2006
Abstract
The organic alcohol 2-methyl-2,4-pentanediol, which is used as cosolvent in the aqueous buffer to induce crystallization of ascorbate oxidase, acts as a competitive inhibitor of ascorbic acid in the oxidase reaction. Glycerol and ethylene glycol show similar, but smaller inhibitory effects. The lower activity exhibited by crystalline ascorbate oxidase with respect to the native enzyme has been shown to depend on the ageing of the proteine solution during crystallization and not by specific effects due to the presence of 2-methyl-2,4-pentanediol.
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