Pyocins are narrow-spectrum bacteriocins synthesized by most isolates of Pseudomonas aeruginosa and are presumed to play a role in niche establishment and protection in mixed populations. P. aeruginosa pyocins can be subdivided into insoluble R and F pyocins and soluble S pyocins. The majority of S-type pyocins (AP41, S1, S2, and S3) cause cell death by DNA cleavage due to an endonuclease C-terminal domain, while pyocins S4 and S5 have tRNAse and pore-forming activities, respectively. Pyocin-producing strains are protected from their own toxin by an immunity protein encoded by a gene usually located downstream the pyocin gene. Pyocin production can be induced by agents that cause DNA damage such as UV irradiation and mitomycin C. In this study, we show that the new small RNA SPA0021, which is transcribed within the pathogenicity island PAPI-1 of P. aeruginosa strain PA14, influences positively the expression of pyocin S3 whose genetic locus comprises two structural genes, pyoS3A and pyoS3I; encoding the killing (S3A) and the immunity (S3I) proteins, respectively. Interestingly, the deletion of SPA0021 gene resulted in increased sensitivity to UV irradiation and to the fluorochinolone antibiotic ciprofloxacin. The degree of UV sensitivity displayed by the PA14 strain lacking SPA0021 was comparable to that of a strain deleted for pyoS3A pyoS3I. These results suggested an involvement of pyocin S3 in DNA damage repair and a regulatory role of SPA0021 on this function.
Small RNA-mediated influence of pyocin S3 production and UV sensitivity in Pseudomonas aeruginosa PA14 / S. Ferrara, S. Carloni, A. Martuscelli, R. Macchi, G. Bertoni. ((Intervento presentato al 31. convegno Microbiology tenutosi a Ravenna nel 2015.
|Titolo:||Small RNA-mediated influence of pyocin S3 production and UV sensitivity in Pseudomonas aeruginosa PA14|
FERRARA, SILVIA (Primo)
CARLONI, SARA (Secondo)
MACCHI, RAFFAELLA ADA (Penultimo)
BERTONI, GIOVANNI (Ultimo)
|Data di pubblicazione:||set-2015|
|Parole Chiave:||Pseudomonas aeruginosa; small RNA; pyocins; DNA damage|
|Settore Scientifico Disciplinare:||Settore BIO/19 - Microbiologia Generale|
Settore BIO/18 - Genetica
|Enti collegati al convegno:||Società Italiana di Microbiologia Generale e Biotecnologie Microbiche|
|Citazione:||Small RNA-mediated influence of pyocin S3 production and UV sensitivity in Pseudomonas aeruginosa PA14 / S. Ferrara, S. Carloni, A. Martuscelli, R. Macchi, G. Bertoni. ((Intervento presentato al 31. convegno Microbiology tenutosi a Ravenna nel 2015.|
|Appare nelle tipologie:||14 - Intervento a convegno non pubblicato|