Biomater ials with a positive impact on neurogenic processes could give a valuable contribution to prospective cell replacement therapies for neurodegenerative diseases. The idea behind it is the in vitro recapitulation of neurogenesis for the substitution of damaged neurons by healthy neurons. In general, the capacity of biomaterials to favour differentiation-promoting effects relies on the cellular competence for mechanotransduction; the perception of biophysical signals (i.e. rigidity and topography) and their conversion into biochemical cellular responses. So far most approaches in the context of neuronal cells were based on the modulation of substrate rigidity. Here we present the possibility to promote neuron differentiation and maturation by appropriate nanorough zirconia surfaces produced by supersonic cluster beam deposition (SCBD). Neonatal rat hippocampal neuronal cells showed accelerated and enhanced maturation and synaptogenesis on these surfaces. This was demonstrated by the earlier and stronger presence of synaptic markers, electrophysiological activity on the single cell and network level and a protein profile confirming an advanced state of neurogenic events, analysed by label-free shotgun proteomics. Congruent to the nature of the biophysical signal input, the proteomic data also suggest a strong involvement of cytoskeleton-and integrin adhesome-related processes. Altogether, our results strongly indicate a promising potential of nanotopographic features of these surfaces in supporting pivotal neuronal differentiation processes.

Nanorough zirconia surfaces promote differentiation and maturation events in neonatal hippocampal neuronal cells / E. Maffioli, C. Schulte, M. Ripamonti, L. Puricelli, C. Piazzoni, E. Sogne, F. Santagata, A. Podestà, C. Lenardi, A. Malgaroli, P. Milani, G. Tedeschi. ((Intervento presentato al 6. convegno Molecular Mechanisms of Neurodegeneration tenutosi a Milano nel 2015.

Nanorough zirconia surfaces promote differentiation and maturation events in neonatal hippocampal neuronal cells

C. Schulte;L. Puricelli;C. Piazzoni;E. Sogne;F. Santagata;A. Podestà;C. Lenardi;P. Milani;G. Tedeschi
2015-05-28

Abstract

Biomater ials with a positive impact on neurogenic processes could give a valuable contribution to prospective cell replacement therapies for neurodegenerative diseases. The idea behind it is the in vitro recapitulation of neurogenesis for the substitution of damaged neurons by healthy neurons. In general, the capacity of biomaterials to favour differentiation-promoting effects relies on the cellular competence for mechanotransduction; the perception of biophysical signals (i.e. rigidity and topography) and their conversion into biochemical cellular responses. So far most approaches in the context of neuronal cells were based on the modulation of substrate rigidity. Here we present the possibility to promote neuron differentiation and maturation by appropriate nanorough zirconia surfaces produced by supersonic cluster beam deposition (SCBD). Neonatal rat hippocampal neuronal cells showed accelerated and enhanced maturation and synaptogenesis on these surfaces. This was demonstrated by the earlier and stronger presence of synaptic markers, electrophysiological activity on the single cell and network level and a protein profile confirming an advanced state of neurogenic events, analysed by label-free shotgun proteomics. Congruent to the nature of the biophysical signal input, the proteomic data also suggest a strong involvement of cytoskeleton-and integrin adhesome-related processes. Altogether, our results strongly indicate a promising potential of nanotopographic features of these surfaces in supporting pivotal neuronal differentiation processes.
Settore BIO/10 - Biochimica
Nanorough zirconia surfaces promote differentiation and maturation events in neonatal hippocampal neuronal cells / E. Maffioli, C. Schulte, M. Ripamonti, L. Puricelli, C. Piazzoni, E. Sogne, F. Santagata, A. Podestà, C. Lenardi, A. Malgaroli, P. Milani, G. Tedeschi. ((Intervento presentato al 6. convegno Molecular Mechanisms of Neurodegeneration tenutosi a Milano nel 2015.
Conference Object
File in questo prodotto:
Non ci sono file associati a questo prodotto.
Pubblicazioni consigliate

Caricamento pubblicazioni consigliate

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2434/327795
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus ND
  • ???jsp.display-item.citation.isi??? ND
social impact